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园艺学报 ›› 2003, Vol. 30 ›› Issue (4): 452-454.

• 研究报告 • 上一篇    下一篇

水杨酸和高温锻炼与葡萄抗热性及抗氧化的关系

王利军 黄卫东 战吉成   

  1. (中国农业大学食品科学与营养工程学院,北京100094)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-08-25 发布日期:2003-08-25

Therm otolerance Related to Antioxidation Induced by in Grape Seednngs

Wang an,Huang Weidong, and Zhan Jicheng

  

  • Received:1900-01-01 Revised:1900-01-01 Online:2003-08-25 Published:2003-08-25

摘要: 对‘京秀’葡萄(Vitis vinifera CV.Jingxiu)高温锻炼和外施水杨酸(salicylic acid,SA)都能显著提高其幼苗的抗热性。高温锻炼1 h,叶片内自由态sA含量急剧升高,其后又迅速下降。在抗热性诱导过程中(高温锻炼12 h或水杨酸喷施后6 h),抗氧化酶系统中的过氧化物酶(POD)、超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)、抗坏血酸过氧化物酶(APX)的活性都明显升高,但过氧化氢酶(CAT)活性下降;高温锻炼或外施SA 1 h,过氧化氢(H2o2)含量急剧升高,之后又迅速下降,可能起着一种信号分子的作用。以上结果说明内源sA可能通过提高抗氧化酶活性参与了高温锻炼过程,外施sA和高温锻炼有相似的提高抗热性机制。

关键词: 葡萄, 水杨酸(SA), 高温锻炼, 抗热性, 抗氧化酶

Abstract:
(CoUpe ofHorticulture,China Agr/cuhura/ ,~/j/ng 100094,Ch/na)
Thennotolerance and antioxidation induced by heat acclimation or exogenous SA treatment were studied in grape(Vitis vinifera CV.Jingxiu)seedlings. e results showed that thermotolerance of grape~ mngs was enhanced by heat acclimation or exogenous SA. en seedlings were acclimated under 38℃ for lh.the content of free SA in grape leaves rose from 3.086 gg‘g FM to 19.081 gg。g FM .then declined to 8.269 gg。g FM after 6 h of heat cclimation.Activities of antioxidant enzymes including superoxide dismutase(SOD),per0 一
dase(POD),glutathione reductase(GR)and ascorbic pemxidase(APX)were elevated,but the activtity of catalase(CAT)Was decreased. e content of hydrogen peroxide(H202)in grape leaves were drastically elevated at lh.then rapidly declined . e results suggested that SA ma y take part in heat acclimation that induced thermo tolerance through induction activity of antioxidative enzymes, SA treatment an d heat acclima tion showed common
mechanism of inducing theromotolerance of plants.

Key words: vinifera, Salicyhc acid, Heat acclimation, Thermotoleranee, Antioxidant nzyme

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