马铃薯晚疫病菌小种特异无毒基因候选表达序列的cDNA-AFLP鉴定

园艺学报 ›› 2005, Vol. 32 ›› Issue (1): 44-48.

马铃薯晚疫病菌小种特异无毒基因候选表达序列的cDNA-AFLP鉴定

郭军;屈冬玉;王晓武;金黎平;谢开云;Rays H. Y. Jiang;Francine Govers

(¹ 中国农业科学院蔬菜花卉研究所, 北京100081; ²Laboratory of Phytopathology, Wageningen University and Graduate School of Experimental Plant Sciences, Binnenhaven 5, 6709 PD Wageningen, The Netherlands)

收稿日期:2004-03-18 修回日期:2004-08-18 出版日期:2005-02-25 发布日期:2005-02-25

Identification of Candidate Expressed Sequences Associated with Race-specific Avirulence Genes by cDNA-Amplified Fragment Length Polymorphism

Guo Jun;Qu Dongyu;Wang Xiaowu;Jin Liping;Xie Kaiyun;Rays H. Y. Jiang;Francine Govers

( ¹ Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ²Laboratory of Phy topathology, Wageningen University and Graduate School of Experim ental Plant Sciences, Binnenhaven 5, 6709 PD Wageningen, The Netherlands)

Received:2004-03-18 Revised:2004-08-18 Online:2005-02-25 Published:2005-02-25

摘要/Abstract

摘要： 利用具有6个无毒基因不同表现型且处于萌发中的静孢子时期的马铃薯晚疫病菌构建4个混合池, 通过cDNA2AFLP分析, 共获得85个与无毒基因相关的差异表达片段, 其中与无毒基因Avr1、Avr2、Avr3-Avr10-Avr11、Avr4 相关的差异表达片段分别为20, 28, 16和21个。将这些差异表达片段对20个晚疫病病菌个体进行无毒基因差异表达验证, 得到1 个无毒基因连锁群Avr3-Avr10-Avr11 的候选片段, 即A11T13Avr3-10-11S157; 无毒基因Avr4的候选片段2个, 即A24T19Avr4S122和A24T24Avr4S142。Blast分析表明: A11T13Avr3210211S157 与马铃薯晚疫病菌萌发孢子囊( germinating sporangium ) EST ( expressed sequence tag) 库中的序列PG001F10.XT7 同源; A24T19Avr4S122 与晚疫病菌萌发中静孢子( germinating cyst) EST库中的PH051G10.XT7部分序列完全一致, 而A24T24Avr4S142与PH051G10. XT7具有98%的同源性。这些结果将促进通过电子克隆并结合RACE (Rapid Amplification of cDNA Ends) 技术获得全长的无毒基因Avr4。

关键词: 马铃薯晚疫病菌, 无毒基因, cDNA-AFLP

Abstract: Through cDNA-AFLP analysis of Four pools with different phenotypes of Six avirulence genes in germinating cysts of Phytophthora infestans, totally 85 candidate transcript derived fragments (TDFs) were obtained, including 20 for Avr1 , 28 for Avr2 , 16 for Avr32A vr102A vr11 , and 21 for A vr4 , respectively. One candidate TDF of linkage genes Avr3-Avr10-Avr11 (A11T13Avr3-10-11S157 ) and two candidate TDFs of A vr4 (A24T19Avr4S122 and A24T24A vr4S142 ) were identified through verification of expression of these TDFs in 20 P. infestans individuals. The results of Blast analysis showed that A11T13A vr3-10-11S157 was homologous with EST G001F101XT7 deposited in the Phytophthora germinating sporangium EST library, A24T19 A vr4S122 was identical with part of EST PH051G101XT7 deposited in the Phytophthora germinating cyst EST library while A24T24A vr4S142 had 98% similarity to
EST PH051G101XT7. That would facilitate cloning of full-length Avr4 gene by in silico cloning along with RACE (Rapid Amplification of cDNA Ends) technique.

Key words: Phytophthora infestans, Racespecific avirulence genes, cDNA-AFLP, Candidate transcript derived fragments

中图分类号:

S532

郭军;屈冬玉;王晓武;金黎平;谢开云;Rays H. Y. Jiang;Francine Govers. 马铃薯晚疫病菌小种特异无毒基因候选表达序列的cDNA-AFLP鉴定[J]. 园艺学报, 2005, 32(1): 44-48.

Guo Jun;Qu Dongyu;Wang Xiaowu;Jin Liping;Xie Kaiyun;Rays H. Y. Jiang;Francine Govers. Identification of Candidate Expressed Sequences Associated with Race-specific Avirulence Genes by cDNA-Amplified Fragment Length Polymorphism[J]. ACTA HORTICULTURAE SINICA, 2005, 32(1): 44-48.

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