https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2003, Vol. 30 ›› Issue (02): 232-235.

• 研究报告 • 上一篇    下一篇

月季切花采后花瓣内肽酶活性的变化

丛日晨;赵喜亭;刘晓辉;高俊平
  

  1. (中国农业大学观赏园艺与园林系,北京100094)
  • 收稿日期:2002-08-10 修回日期:2003-02-13 出版日期:2003-04-25 发布日期:2003-04-25

Difference of Endopeptidase Activity in Petals between Two Cut Rose Cultivars with Diferent Tolerance to W ater Deficit Stress during Flower Opening and Senescence

Cong Richen;Zhao Xiting; Liu Xiaohui; Gao Junping
  

  1. (Department of Ornamental Horticulture and landscape Architecture,China Agricultural University,Beijing 100094,China )
  • Received:2002-08-10 Revised:2003-02-13 Online:2003-04-25 Published:2003-04-25

摘要: 以切花月季耐失水胁迫品种‘萨蔓莎’和中度耐失水胁迫品种‘贝拉米’为试材,研究了采后开花衰老进程中可溶性蛋白质和游离氨基酸含量及内肽酶活性和种类的变化。结果表明,两品种可溶性蛋白质和游离氨基酸含量及内肽酶活性在开花衰老进程中的变化趋势基本一致,但是在量上差异非常明显。可溶性蛋白质含量‘萨蔓莎’低于‘贝拉米’;pH6时,内肽酶活性‘萨蔓莎’低于‘贝拉米’,pH10时相反。两品种花瓣中都存在巯基蛋白酶和丝氨酸蛋白酶。切花月季的失水胁迫耐性可能与其内肽酶活性有关。

关键词: 月季, 切花, 开花, 衰老, 内肽酶

Abstract: Cut rose(Rosa hybris )‘Samantha’,a cultivar with strong tolerance to water deficit stress,and‘Belami’,a cultivarwith middle range tolerance weI'e used to investigate the cha ses of contents of soluble protein and free amino acid, and endopeptidase activity in petals during flower opening and senescence. The results showed that obvious differences between the two cultivars were obtained in contents of solub le protein and free amino acid,an dendopeptidase activity during flower ope ning and senescence,although the salTle change patterns were obtained .Soluble protein content was lower in ‘Samantha’tllall in ‘Belami’.Endope ptidase activity Was much higher at pH 10,although it Was lower at pH 6 in‘Samantha’than in ‘Belami’.However,it Was confirmed in petals of the two cultivars that there were two kinds of endopeptidase:mercapto proteinase an d serine acid proteinase.The results above suggested that the diference of endopeptidase activity between two cultivars might be related to their tolerance to water de6c stress.

Key words: Rose, Cut rose, Flower opening, Flower senescence, Endope ptidase

中图分类号: