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园艺学报 ›› 2003, Vol. 30 ›› Issue (02): 236-238.

• 研究报告 • 上一篇    下一篇

悬铃木叶片植株再生系统的建立

范国强;蒋建平;贺窑青;李 锋
  

  1. (河南农业大学泡桐研究所,郑州450002)
  • 收稿日期:2002-04-30 修回日期:2002-08-06 出版日期:2003-04-25 发布日期:2003-04-25

In Vitro Efficient Plant Regeneration th Platanus orientalis L. Leaves asExplants

Fan Guoqiang;Jiang Jianping;He Yaoqing; Li Feng
  

  1. (Institute of Paulownia,Henan Agricultural University,Zhengzhou 4501302,China)
  • Received:2002-04-30 Revised:2002-08-06 Online:2003-04-25 Published:2003-04-25

摘要:

以悬铃木实生苗叶片及其诱导的愈伤组织为材料建立植株再生系统。结果表明,WPM可作为悬铃木叶片体外再生系统的基本培养基。从基部向上数第5、6、7片叶诱导芽最适培养基为WPM+IBA 0.1(单位mg· L-1, 下同)+BA 3.0~2.0;对第5片叶在WPM+IBA 0.6+BA 8.0上诱导出的愈伤组织,再进行芽诱导的最适培养基为WPM+IBA 0.1+BA 4.8;幼苗根诱导的最适培养基为1/2 Ms+IBA 0.1~0.5。

关键词: 悬铃木, 叶片, 植株, 再生

Abstract: This paper dealt with in vitro eficient o~ant regeneration on WPM media with 0.6(mg· L-1,the same below)IBA and 8.0 BA,using Platanus orientalis L.1eaves and their calli as explanm.The results indicated that WPM might be the basic medium for the plant regeneration,and that bud inductionⅡ ia of the 5th,6th,7th leaves (from the bases of seedlings) +0.1 IBA+3.0—2.0 BA,and the 5th leaf cam induced on +0.6IBA+8.0 BA medium was +0.1 IBA+4.8 BA.Root induction medium was 1/2 MS+0.1—0.5IBA.These results might provide reference to cell and gene engineering manipulations and quick propagations of Phuanus orienitalis

Key words: Phuanus orienitalis, Leaf, Plant, Regeneration

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