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园艺学报 ›› 2005, Vol. 32 ›› Issue (1): 39-43.

• 研究论文 • 上一篇    下一篇

马铃薯脱外壁花粉的制备与人工萌发

吴旺泽;王蒂;王清;彭晓莉   

  1. (甘肃农业大学农学院, 兰州730070)
  • 收稿日期:2004-03-11 修回日期:2004-08-12 出版日期:2005-02-25 发布日期:2005-02-25

Preparation and Artificial Germination of De-exined Pollen in Potato (Solanum tuberosum L. )

Wu Wangze;Wang Di;Wang Qing;Peng Xiaoli   

  1. (College of Agronomy , Gansu Agriculture University, Lanzhou 730070, China)
  • Received:2004-03-11 Revised:2004-08-12 Online:2005-02-25 Published:2005-02-25

摘要: 将3个马铃薯普通栽培种( Solanum tuberosum L. ) ‘陇薯3号’、‘Favorita’和‘Shapody’的花粉先经低温饥饿处理, 再低温水合、高温热激和渗激, 分离出大量具有活力的脱外壁花粉, 花粉最高脱壁率达34.5%。脱外壁花粉在K3 培养基+ 15% PEG + 5%蔗糖+ 0.01% H3BO3 + 0.01% LH + 100mg/L PVP的萌发液中萌发率高达38.3%。试验建立了三步冲击法制备马铃薯脱外壁花粉的方法, 并讨论了外壁在花粉萌发与受粉中的生物学意义。

关键词: 马铃薯, 花粉, 脱外壁花粉, 人工萌发

Abstract: Pollen grains of three cultivars‘Longshu 3’, ‘Favorita’and ‘Shapody’of potato were treated following a three2step p rocedure: 1. Starvation treatment at 4 - 7℃ for 3 - 5 d; 2. Hydration at 4℃ for 20 h; 3. Heat shock at 36℃ for 20 - 100 min and osmotic shock for 40 min. After the three2step treatment the exine could be detached along the germination furrowswith the detachment rate of 34.5%. The germination rate of the nake pollen of cultivars potato could reach 38.3% in a modified medium containing 15% PEG (MW 8000) , 5% sucrose, 0.01% H3BO3 , 0.01% LH, 100 mg/L PVP and K3'salts ( pH 8.5). The results showed the three-step procedure established in this experiment was a useful tool for detaching the exine of potato pollen and understanding the biological role of exine in pollen germination and pollination.

Key words: Potato, Pollen, De-exined pollen, Artificial pollen germination

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