关键词: 甘蓝, 细胞质雄性不育, 分子分型, 花器官形态, 核背景

Abstract:

Fifteen cytoplasmic male sterile materials in Brassica oleracea was identified by amplified special cytoplasmic male sterile associated gene，all of the CMS materials were crossed and backcrossed with different inbred-lines for several successive generations to investigated the morphology response of flower to nuclear background. The result showed that：Ogu CMS take up the most part in the materials investigatied. Fourteen materials of 15 belong to this class. Only No.78 belong to Nap CMS. The response of Nap CMS to different nuclear background showed three types. The first type was similar with original material or show negative response；The second type was positive response；The third type was complicated，varying with floret parts. The Ogu CMS materials either showed a weak positive response to nuclear background or keep unchanged comparing to original material. Morphology response of different source of Ogu CMS to the same nuclear background was varied with unclear background. The inbred-line K1，N4 have similar impact on different cytoplasmic male sterile，but 37，F1 and G7 have different effect on different sources of cytoplasmic male sterile in flower morphology.

Key words: Brassica oleracea, cytoplasmic male sterile, molecular identification, flower organs morphology, nuclear background