国产石斛属植物亲缘关系的AFLP分析

园艺学报 ›› 2007, Vol. 34 ›› Issue (6): 1569-1574.

国产石斛属植物亲缘关系的AFLP分析

白音^1,2*;包英华¹;王文权²;姜丽丽³;阎玉凝²

（¹韶关学院英东生物工程学院，广东韶关 512005；²北京中医药大学中药学院，北京 100102；³中国科学院植物研究所，北京 100093）

收稿日期:2007-05-31 修回日期:2007-08-24 出版日期:2007-12-25 发布日期:2007-12-25

Analysis of the Phylogenetic Relationship of Dendrobium in Chinaby AFLP Technique

BAI Yin^{1, 2*}, BAO Ying-hua¹, WANG Wen-quan², JIANG Li-li³, and YAN Yu-ning²

(¹Yingdong College of Bioengineering, Shaoguan University, Shaoguan, Guangdong 512005, China; ²School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China; ³Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China)

Received:2007-05-31 Revised:2007-08-24 Online:2007-12-25 Published:2007-12-25

摘要/Abstract

摘要： 采用AFLP技术对我国38种石斛属植物进行亲缘关系分析，从64对选择性引物中筛选出8对引物组合，共扩增出1 644个位点，其中多态性位点1 639，占总扩增位点的99.7%，说明我国石斛属植物遗传多态性水平很高。聚类分析结果表明，石斛组和黑毛组并没有形成独立的分支；剑叶组和圆柱叶组植物共同构成一个分支，并与其它组之间的相似度较低，说明该两组与其它组之间的亲缘关系较远；禾叶组、草叶组和叉唇组均形成独立的分支，有向各自的演化方向发展的趋势。国产石斛属植物亲缘关系比较复杂，并非是一个单系类群，可能存在一些并系类群或多系类群。

关键词: 石斛属, 亲缘关系, AFLP

Abstract: Thirty-eight species of Dendrobium were analyzed phylogenetically using AFLP technique. Eight pairs of primers combination were screened from 64 pairs of primers combination and generated 1 644 amplified loci, 1 639 of which were polymorphic loci which accounted for 99.7% in the total amplified loci. It showed that the generation diversity standard of Dendrobium was very high in our country. Cluster analysis showed that Sect. Dendrobium and Sect. Formosae did not form an independent branch, Sect. Aporum and Sect. Strongyle were consisted of a common branch. They had a lower similarity with another section. Sect. Grastidium, Sect. Stachyobium and Sect. Stuposa formed an independent branch. They had a different evolution direction. Genetic relationship in domestic Dendrobium was much more complicated, and they were not a monophyletic group in nature, but a paraphyletic or polyphyletic group.

Key words: Key words: Dendrobium, Phylogenetic relationship, AFLP

中图分类号:

S 682.31

白音;包英华;王文权;姜丽丽;阎玉凝. 国产石斛属植物亲缘关系的AFLP分析[J]. 园艺学报, 2007, 34(6): 1569-1574.

BAI Yin;;BAO Ying-hua;WANG Wen-quan;JIANG Li-li;and YAN Yu-ning. Analysis of the Phylogenetic Relationship of Dendrobium in Chinaby AFLP Technique[J]. ACTA HORTICULTURAE SINICA, 2007, 34(6): 1569-1574.

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