辣椒属种间杂交及杂种鉴定研究

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 883-888.

辣椒属种间杂交及杂种鉴定研究

程志芳¹; 钱春桃¹; 陈学军^{1, 2}; 陈劲枫^1*

(¹作物遗传与种质创新国家重点实验室, 南京农业大学园艺学院, 南京210095; ² 江西省农业科学院蔬菜花卉研究所, 南昌330200)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25
通讯作者: 陈劲枫

Interspecific Hybridization and Identification of Hybrid in Capsicum

CHENG Zhi-fang¹;QIAN Chun-tao¹;CHEN Xue-jun^1,2; CHEN Jin-feng^1*

(¹ State Key Laboratory of Crop Genetics and Germ plasm Enhancement, College of Horticulture, Nanjing Agricultural University,Nanjing 210095, China; ² Institute of Vegetable and Flower, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25
Contact: CHEN J in-feng

摘要/Abstract

摘要：

通过辣椒属5个栽培种(Capsicum annuum, C. chinense, C. f rutescens, C. baccatum 和C. pubescens) 种间正反杂交试验, 获得了3株C. baccatum ×C. chinense (H_bc ) 和7株C. annuum ×C. chinense
(H_ac ) 种间杂种植株。除H_ac-7单株表型异常外, 其他杂种F1植株均生长正常。H_ac、H_bc花粉可染率分别为
13.9%和23.8% , 显著低于其双亲。同一组合内单株间花粉育性、形态性状和同工酶电泳图谱表现一致,形态学观察和过氧化物同工酶电泳分析结果均验证了种间杂种的真实性。

关键词: 辣椒, 种间杂种, 过氧化物酶

Abstract: Three hybrid p lants from the combination of Capsicum baccatum ×C. chinense (H_bc ) and seven hybrid plants of C. annuum ×C. chinense (H_ac ) were obtained through recip rocal interspecific hybridiza
tions among the five cultivated species C. annuum, C. chinense, C. frutescens, C. baccatum, and C. pu
bescens. Excep t for the H_ac-7 which exhibited virus-like symp toms, all the other hybrid p lants grew normally.
The pollen stainability percentages of H_ac and H_bc were 13.9% and 23.8%, respectively, and were significant
ly lower than those of their corresponding parents. The pollen stainability percentage, morphological characters
and band patterns of isozyme in the hybrid p lantswere identicalwithin each combination. The nature of hybrid
was confirmed by morphological characteristics and isozyme (peroxidase) analysis.

Key words: Capsicum, Interspecific hybrid, Peroxidase isozyme

程志芳;钱春桃;陈学军;;陈劲枫. 辣椒属种间杂交及杂种鉴定研究[J]. 园艺学报, 2007, 34(4): 883-888.

CHENG Zhi-fang;QIAN Chun-tao;CHEN Xue-jun;CHEN Jin-feng. Interspecific Hybridization and Identification of Hybrid in Capsicum[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 883-888.

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