部分芍药种质资源的RAPD分析

园艺学报 ›› 2007, Vol. 34 ›› Issue (5): 1321-1326.

部分芍药种质资源的RAPD分析

郭先锋^1* ; 王莲英²

（¹山东农业大学林学院，山东泰安 271018；²北京林业大学园林学院，北京 100083）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-10-25 发布日期:2007-10-25

Genetic Relationship of Partial Peony Germplasm Resources with RAPD Markers

GUO Xian-feng^1*; WANG Lian-ying²

¹College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, China; ²College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

摘要/Abstract

摘要： 对5种野生芍药和26个栽培芍药品种的亲缘关系进行了RAPD分析。17条引物在所有供试样本中共扩增出153条反应带，其中多态带149条。对RAPD扩增结果采用UPGMA法进行聚类分析，在遗传相似度0.32处，块根芍药(Paeonia anomala)被单独分为一类，其它30份材料归为另一类；在遗传相似度0.54处，川赤芍(P.veitchii)、草芍药(P.obovata)和美丽芍药(P.mairei)3个野生种先后从30份材料中分别单独分离出来，而野生种芍药(P. lactiflora)则与所有的栽培品种聚为一类。该结果表明26个品种间的遗传背景相对一致，并与野生种芍药的遗传基础最为近密，与其它4种野生芍药的遗传差异则较大。按照与栽培品种亲缘关系由近到远的次序，4个野生近缘种依次排布为：美丽芍药、草芍药、川赤芍和块根芍药。研究结果从分子水平揭示出中国芍药品种群遗传背景狭窄，利用野生近缘种改良现有品种尚具有广阔空间。

关键词: 芍药, 种质资源, 亲缘关系, RAPD

Abstract: Relationship among 5 wild species and 26 cultivars were analyzed using RAPD（Random amplified polymorphic DNA）markers. One hundred and fifty three amplified fragments were detected by applying 17 10-mer primers, among them 149 fragments were polymorphic. Cluster analysis was then performed by UPGMA method. In the dendrogram based on the genetic similarity coefficients, Paeonia anomala were separated from other 30 samples at GSC(Genetic Similarity Coefficient) of 0.32; And at GSC of 0.54, P.veitchii, P.obovata and P.mairei were individually separated, while P.lactiflora was still linked together with all of the cultivars. The result suggested high genetic homogeneity between the cultivars and P.lactiflora. Comparatively, the other 4 wild species showed distant genetic relationship with the cultivars, and the order based on the GSC from close to distant was arranged as P.mairei, P.obovata,P.veitchii and P.anomola. This molecular study revealed the narrow genetic background of the cultivated peonies native to China, so there exists broad space of improving the cultivars with wild species. 

Key words: Peony, Germplasm resource, Relationship, RAPD

郭先锋;王莲英. 部分芍药种质资源的RAPD分析[J]. 园艺学报, 2007, 34(5): 1321-1326.

GUO Xian-feng;WANG Lian-ying. Genetic Relationship of Partial Peony Germplasm Resources with RAPD Markers[J]. ACTA HORTICULTURAE SINICA, 2007, 34(5): 1321-1326.

[1]	朱炜, 曹津津, 陈曦, 张葳, 孙榕泽, 朱绍才, 赵家庚, 崔雅琦, 王宇暄, 于晓南. 芍药新品种‘粉罗裙’‘千重粉黛’‘笑春风’和‘碧叶粉荷’[J]. 园艺学报, 2023, 50(2): 457-458.
[2]	史小华, 马广莹, 张佳平, 朱开元, 王小斌. 芍药新品种‘江南小新’[J]. 园艺学报, 2022, 49(S2): 181-182.
[3]	史小华, 张佳平, 马广莹, 王小斌, 朱开元. 芍药新品种‘江南月明’[J]. 园艺学报, 2022, 49(S2): 183-184.
[4]	王小斌, 张栋, 史小华, 李丹青, 张润龙, 邵灵梅, 许曈, 夏宜平, 张佳平, . 芍药新品种‘紫心’[J]. 园艺学报, 2022, 49(S1): 115-116.
[5]	陈曦, 刘志洋, 门万杰, 张冬, 汪磊. 芍药新品种‘紫美人’[J]. 园艺学报, 2022, 49(S1): 117-118.
[6]	吴凡, 江俊浩, 卢山, 张楠, 邱帅, 魏建芬, 沈柏春. 中国绣球属种质资源及其利用研究进展[J]. 园艺学报, 2022, 49(9): 2037-2050.
[7]	吕正鑫, 贺艳群, 贾东峰, 黄春辉, 钟敏, 廖光联, 朱壹, 袁开昌, 刘传浩, 徐小彪. 猕猴桃种质资源表型性状遗传多样性分析[J]. 园艺学报, 2022, 49(7): 1571-1581.
[8]	李平平, 张祥, 刘雨婷, 谢志和, 张芮豪, 赵凯, 吕俊恒, 王梓然, 文锦芬, 邹学校, 邓明华. 辣椒63份种质果皮颜色与呈色物质的关系[J]. 园艺学报, 2022, 49(7): 1589-1601.
[9]	张萌, 单玉莹, 杨业波, 翟飞飞, 王兆山, 巨关升, 孙振元, 李振坚. 中国石斛属植物遗传资源的AFLP分析[J]. 园艺学报, 2022, 49(6): 1339-1350.
[10]	蔡志翔, 严娟, 宿子文, 徐子媛, 张明昊, 沈志军, 杨军, 马瑞娟, 俞明亮. 不同类型桃种质资源主要酚类物质含量评价[J]. 园艺学报, 2022, 49(5): 1008-1022.
[11]	张世才, 李怡斐, 王春萍, 杨小苗, 黄启中, 黄任中. 辣椒种质尖孢炭疽菌抗性鉴定与评价[J]. 园艺学报, 2022, 49(4): 885-892.
[12]	徐婉, 林雅君, 赵荘, 周庄. 兰属植物资源与育种研究进展[J]. 园艺学报, 2022, 49(12): 2722-2742.
[13]	籍凤娇, 马燕, 亓帅, 郭先锋, 陈俊强. 芍药PlSVP基因的克隆及其花期调控功能分析[J]. 园艺学报, 2022, 49(11): 2367-2376.
[14]	吴致君, 李伟, 王兴华, 张成, 蒋勋, 黎盛, 罗理勇, 孙康, 曾亮. 基于SLAF-seq的茶组子房室数不稳定居群种质亲缘关系分析[J]. 园艺学报, 2022, 49(11): 2455-2470.
[15]	邓秀新. 中国柑橘育种60年回顾与展望[J]. 园艺学报, 2022, 49(10): 2063-2074.