西南凤尾蕨（Pteris wallichiana Agardh.）发育过程及其孢子的无菌繁殖

园艺学报 ›› 2008, Vol. 35 ›› Issue (1): 94-98.

西南凤尾蕨（Pteris wallichiana Agardh.）发育过程及其孢子的无菌繁殖

张开梅^1,2;石雷^1*;李东¹;张宪春¹

（¹中国科学院植物研究所，北京 100093；²中国科学院研究生院，北京 100049）

收稿日期:2007-07-06 修回日期:2007-11-22 出版日期:2008-01-25 发布日期:2008-01-25
通讯作者: 石雷

Development Process and Spore Sterile Culture of Pteris wallichiana Agardh.

ZHANG Kai-mei^1,2, SHI Lei^1*, LI Dong¹, and ZHANG Xian-chun¹

（¹Institute of Botany, the Chinese Academy of Sciences, Beijng 100093, China；²Graduate University of Chinese Academy of Sciences, Beijing 100049, China）

Received:2007-07-06 Revised:2007-11-22 Online:2008-01-25 Published:2008-01-25

摘要/Abstract

摘要： 研究了西南凤尾蕨的发育过程和孢子的无菌培养方法。结果如下：孢子萌发为书带蕨型，配子体发育为水蕨型。1/2MS培养基中萌发率可达83.3%；MS培养基中配子体成苗率可达55.4%；试管苗移栽成活率可达80%以上。认为利用孢子的无菌繁殖方法进行西南凤尾蕨的规模化生产具有可行性。

关键词: 西南凤尾蕨, 孢子, 无菌培养

Abstract: The development process and spore sterile culture of Pteris wallichiana Agardh. were studied respectively. The results showed that the spore germination was of the Vittaria-type while the gametophyte development was of the Ceratopteris-type. The maximum germination rate was obtained in 1/2MS medium, which reached 83.3%; the highest rate of gametophyte to sporophyte was achieved in MS medium, which reached 55.4%; the transplant survival rate of sporophyte in vitro reached above 80%. The research indicates that the utilization of the spore sterile culture of P. wallichiana for its large-scale production is possible.

Key words: Pteris wallichiana Agardh., spore, sterile culture

中图分类号:

S 68

张开梅;石雷;李东;张宪春. 西南凤尾蕨（Pteris wallichiana Agardh.）发育过程及其孢子的无菌繁殖[J]. 园艺学报, 2008, 35(1): 94-98.

ZHANG Kai-mei;SHI Lei;LI Dong;and ZHANG Xian-chun. Development Process and Spore Sterile Culture of Pteris wallichiana Agardh.[J]. ACTA HORTICULTURAE SINICA, 2008, 35(1): 94-98.

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