洋葱T型细胞质雄性不育相关基因的结构与表达模式研究

园艺学报 ›› 2010, Vol. 37 ›› Issue (4): 631-636.

洋葱T型细胞质雄性不育相关基因的结构与表达模式研究

吴海涛1,3，王建军1,2，侯喜林1,2,*，刘洪炯3，马蓉莉3

（1南京农业大学作物遗传与种质创新国家重点实验室，南京210095；2农业部南方蔬菜遗传改良重点开放实验室，南京210095；3山西省农业科学院蔬菜研究所，太原030031）

收稿日期:2010-01-15 修回日期:2010-03-25 出版日期:2010-04-25 发布日期:2010-04-25
通讯作者: 侯喜林

Studies on Structure and Expression Pattern of Chimeric Genes Associated with Onion T-type Cytoplasmic Male Sterility

WU Hai-tao1,3，WANG Jian-jun1,2，HOU Xi-lin1,2,*，LIU Hong-jiong3，and MA Rong-li3

（1State Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjing Agriculture University，Nanjing 210095，China；2Key Laboratory of Southern Vegetable Crop Genetic Improve，Ministry of Agriculture，Nanjing 210095，China；3Vegetable Institute，Shanxi Academy of Agricultural Sciences，Taiyuan 030031，China）

Received:2010-01-15 Revised:2010-03-25 Online:2010-04-25 Published:2010-04-25
Contact: HOU Xi-lin

摘要/Abstract

摘要： orfA501标记与洋葱细胞质雄性不育表型之间的完全符合引起研究者对其所在基因结构的兴趣。试验通过锚定PCR方法获得了orfA501的侧翼序列，明确了其在洋葱T型细胞质雄性不育线粒体基因组中的位置；采用RT-PCR方法研究了orfA501及其相连基因的转录模式。结果表明：洋葱T型细胞质雄性不育线粒体基因组中orfA501 的5′ 端与coxⅠ基因紧密相连，二者相隔93个碱基；orfA501的3′端是由atp9、atp6、atp1以及nad4等基因片段构成的一个复杂序列；在洋葱T型细胞质雄性不育系花药中，orfA501与coxⅠ基因分别转录，且orfA501对coxⅠ的转录具有正调控的作用。

关键词: 洋葱, 细胞质雄性不育, orfA501

Abstract: The perfect coincidence of orfA501-marker and the phenotype of onion cytoplasmic male sterile has arose interesting in researchers. In this experiment，flanking sequences of orfA501 in mitochondrial genome of onion T-type cytoplasmic male sterility was obtained successfully by anchored PCR，and expression pattern of the chimeric gene was analysed by real time PCR. The result showed that：It was cytochrome c oxidase subunit I（coxⅠ）gene who flanked 5′ of orfA501，and a complicated sequence composed by fractions of atp9，atp6，atp1 and nad4 located in the 3′ region of orfA501. Though there were 93 bases between orfA501 and coxⅠ，they transcripted seperately in the anthors of T-type cytoplasmic male sterility，and orfA501 seemed to play an up-regulator factor for the transcription of coxⅠ.

Key words: onion, Allium cepa L., cytoplasmic male sterility, orfA501

中图分类号:

S 633.2

吴海涛;王建军;侯喜林;;刘洪炯;马蓉莉. 洋葱T型细胞质雄性不育相关基因的结构与表达模式研究[J]. 园艺学报, 2010, 37(4): 631-636.

WU Hai-tao;WANG Jian-jun;HOU Xi-lin;;LIU Hong-jiong;and MA Rong-li. Studies on Structure and Expression Pattern of Chimeric Genes Associated with Onion T-type Cytoplasmic Male Sterility[J]. ACTA HORTICULTURAE SINICA, 2010, 37(4): 631-636.

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