关键词: 猕猴桃, 矮型性状, EST序列, SSR标记

Abstract: High effective simple sequence repeats（SSR）marker based on expressed sequence tags （EST）and the method of bulked segregant analysis（BSA）on the automated fluorescent-labeled system（ABI3130）were employed to screen the molecular marker linked to dwarf gene in kiwifruit （Actinidia spp.） from the F1 separating progenies obtained from the cross between dwarf cultivar ‘Ganmi 5’（♀）and normal cultivar‘Fengxiong 2’（♂）of Actinidia chinensis Planch. Eighty-five pairs of fluorescent primers were designed and screened in the present study. The results showed that a polymorphic band of 285 bp amplified by primer EST-Ad042 was occured both in the dwarf parent and in the dwarf cultivars of separating DNA pools but not in normal cultivar. Furthermore，the polymorphic band had been approved by stochastic amplification in the F1 separating progenies including dwarf and normal genotypes，respectively. The genetic distance between the EST-SSR marker and dwarf gene could be 8.8 cM by genetic linkage analysis. The specific molecular marker of kiwifruit could be effectively mined by fluorescent EST-SSR primers，and the fluorescent primer EST-Ad042 could be utilized to identify its dwarf plant. The screening of molecular markers linked to dwarf gene in Actinidia chinensis Planch had been provided a basis for the studies of dwarf gene mapping and cloning in kiwifruit breeding program.

Key words: Actinidia, kiwifruit, dwarf character, EST sequence, SSR marker