https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2007, Vol. 34 ›› Issue (5): 1243-1248.

• 观赏植物 • 上一篇    下一篇

85个大菊品种遗传关系的ISSR分析

缪恒彬;陈发棣*;赵宏波   

  1. (南京农业大学园艺学院, 南京 210095)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-10-25 发布日期:2007-10-25

Genetic Relationship of 85 Chrysanthemum〔Dendranthema×grandiflora(Ramat.) Kitamura〕Cultivars Revealed by ISSR Analysis

MIAO Heng-bin;CHEN Fa-di;ZHAO Hong-bo
  

  1. (College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

摘要: 采用ISSR-PCR分子标记技术对大菊85个品种的遗传多样性进行了分析。从77条ISSR引物中筛选出22条引物分别对供试材料基因组DNA进行扩增,共获得160条清晰可辨谱带,其中多态性带148条,多态位点百分率为92.5%,多态性较高。POPGENE软件计算结果表明,85个大菊品种平均有效等位基因数为1.6013,平均Nei's 基因多样性指数为0.3485,平均Shannon信息指数为0.5162;应用SPSS软件计算得到各品种间的Jaccard相似系数介于0.294~0.802之间。UPGMA法将85个品种分成6个类群,聚类结果与品种瓣型相关。

关键词: 菊花, ISSR, 遗传关系

Abstract: ISSR-PCR was used to detect the genetic diversity and relationship of 85 chrysanthemum cultivars. ISSR fingerprinting amplified by 22 ISSR primers revealed a total number of 160 unambiguous bands, of which 148 ones were polymorphic and the polymorphism frequency was 92.5%. As analyzed by POPGENE 1.31, the average value of effective number of alleles, Nei's gene diversity and Shannon's information index were 1.6013, 0.3485 and 0.5162, respectively. As analyzed by SPSS 11.0, the similarity coefficient between cultivars ranged from 0.294 to 0.802. These 85 chrysanthemum cultivars were divided into six groups by UPGMA (unweighted pair group method with arithmetic average) based on Jaccard coefficient. The genetic relationship of 85 cultivars was related to their petal types.

Key words: Dendranthema×grandiflora, ISSR, Genetic relationship