桃花粉可育基因连锁的RAPD 标记与SCAR标记的转化

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 865-870.

桃花粉可育基因连锁的RAPD 标记与SCAR标记的转化

王　成¹;曹后男^1,3;宗成文¹;赵成日²;庄得凤¹;朴日子¹;赵　恺¹

(¹ 延边大学农学院, 吉林龙井133400; ²歧阜大学应用生物科学部, 日本歧阜5011193)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25

Identif ication of RAPD Marker Linked to Pollen Fertility Gene and Conversion to SCARMarker in Peach

WANG Cheng¹;CAO Hou-nan^1,3;ZONG Cheng-wen¹;ZHAO Cheng-ri²;ZHUANG De-feng¹;P IAO Ri-zi¹;ZHAO Kai¹

(¹Agricultural College, Yanbian University, Longjing, Jilin 133400, China; ² Faculty of Applied Biological Sciences, Gifu University, Gifu 5011193, Japan)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

摘要/Abstract

摘要： 桃品种以重阳红(花粉不育) ×大久保(花粉可育) 的52株F₁代群体为试材, 应用RAPD
技术, 结合集群分组分析法(Bulked Segregate Analysis, BSA) 构建花粉可育/不育基因池, 利用180个
随机引物, 筛选在花粉可育基因池中稳定扩增的一个RAPD标记OPW03-900。经过重复性验证和群体单
株验证, 该标记仅在花粉可育个体(重组型除去) 中出现, 与桃花粉可育/不育位点的连锁距离为5.80
cM。将该特异性片段回收、克隆、测序, 并设计一对特异SCAR引物, 再对F1代个体进行PCR扩增, 发
现该特异带的位点及重组型个体的数目与RAPD扩增结果一致, 片段大小为906 bp, 命名为SCW03-906,表明RAPD标记已成功转化为与桃花粉可育基因连锁的SCAR标记。该序列已被GenBank收录, 登录号为DQ659676。

关键词: RAPD标记, BSA, SCAR标记, 花粉可育基因

Abstract: In this study, a RAPD marker (OPW03-900) linked to pollen fertility gene was screened
from 180 RAPD arbitrary p rimers in 52 F₁ individuals from the cross‘Chongyanghong’ ×‘Okuba’, pollen
fertility/ sterility poolwas constructed respectively by using RAPD technique and BSA method. After repeating
tests and checking up individuals of population, thismarker could only be amp lified in the pollen fertility indi
viduals ( excep t recombinants) , the linkage distance was 5.80 cM with the pollen fertility/ sterility locus. This
band was collected and sequenced to synthesize a pair of SCAR p rimerwhich was used to amp lify the individu
als of the F1 p rogeny, the locus of the special band and the number of recombinantswere the same as the result
of RAPD amp lification, the size of thismarkerwas 906 bp, which was designated as SCW03-906, indicating
the RAPD marker has been successfully converted into a SCAR markerwhich linked to pollen fertility gene in
peach. This sequence was adop ted by GenBank, the logging number wasDQ659676.

Key words: RAPD marker, BSA, SCAR marker, Pollen fertility gene

王　成;曹后男;宗成文;赵成日;庄得凤;朴日子;赵　恺. 桃花粉可育基因连锁的RAPD 标记与SCAR标记的转化[J]. 园艺学报, 2007, 34(4): 865-870.

WANG Cheng;CAO Hou-nan;ZONG Cheng-wen;ZHAO Cheng-ri;ZHUANG De-feng;P IAO Ri-zi;ZHAO Kai. Identif ication of RAPD Marker Linked to Pollen Fertility Gene and Conversion to SCARMarker in Peach[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 865-870.

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