扇叶铁线蕨孢子无菌繁殖技术研究

园艺学报 ›› 2010, Vol. 37 ›› Issue (3): 457-464.

扇叶铁线蕨孢子无菌繁殖技术研究

吴华;袁丽萍;王洋;陈龙清

(华中农业大学园艺林学学院，武汉 430070)

收稿日期:2009-07-02 修回日期:2010-03-08 出版日期:2010-03-25 发布日期:2010-03-25

Studies on in Vitro Culture of Adiantum flabellulatum from Spores

WU Hua;YUAN Li-ping;WANG Yang;CHEN Long-qing

(College of Horticulture and Forestry Science，Huazhong Agricultural University，Wuhan 430070，China)

Received:2009-07-02 Revised:2010-03-08 Online:2010-03-25 Published:2010-03-25

摘要/Abstract

摘要： 研究了扇叶铁线蕨（Adiantum flabellulatum）发育过程和不同因素对孢子、原叶体无菌培养
的影响。结果表明：孢子萌发为书带蕨型，原叶体发育为铁线蕨型；孢子在无光条件下不能萌发；适当时间的低温冷藏有利于孢子萌发；播种20 d 后，1/4MS 培养基中孢子萌发率最高，可达22.7%；在培养基中添加15 ~ 45 g · L^-1 的蔗糖有利于孢子的萌发，蔗糖有利于分生区的发育和性器官的分化；MS + 30 g · L^-1蔗糖的培养基中，原叶体的成苗率可达48.3%；外源生长素不利于原叶体的成苗，但NAA 0.5 mg · L^-1 促进原叶体的离体增殖；用无菌沙瓶做试管苗炼苗基质后的移栽成活率可达90%。

关键词: 扇叶铁线蕨, 孢子, 萌发

Abstract: The development process of spores of Adiantum flabellulatum in sterile culture condition and effects of different factors on spore germination and gametophyte development of A. flabellulatum were studied. The results showed that the spore germination was of the Vittaria-type and the gametophyte
development was of the Adiantum-type. Spore could not geminate in darkness. Proper time of deepfreeze was good for spore germination rate. After 20 days of spore sowing, the maximum germination rate was obtained in 1/4MS medium, which reached 22.7%; additional 15–45 g · L^-1 sucrose in culture medium were benefit spore germination，and sucrose was beneficial to gametophyte sexual differentiation；the sporophyte formation rate could reached 48.3% in MS + 30 g · L^-1 medium；additional auxins were not beneficial to sporophyte formation，but additional NAA 0.5 mg · L^-1 could promote gametophyte multiplication；sporophyte accommodation in sterile sand bottles could support a high alive rate about 90%.

Key words: Adiantum flabellulatum, spore, germination

中图分类号:

S 682.35

吴华;袁丽萍;王洋;陈龙清. 扇叶铁线蕨孢子无菌繁殖技术研究[J]. 园艺学报, 2010, 37(3): 457-464.

WU Hua;YUAN Li-ping;WANG Yang;CHEN Long-qing. Studies on in Vitro Culture of Adiantum flabellulatum from Spores[J]. ACTA HORTICULTURAE SINICA, 2010, 37(3): 457-464.

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