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园艺学报 ›› 2010, Vol. 37 ›› Issue (3): 355-362.

• 果树 • 上一篇    下一篇

高温对桃花粉发育及小孢子产生的影响

叶正文;杜纪红;苏明申;李六林;张绍铃

  

  1. 1 南京农业大学园艺学院,南京 210095;2 上海市农业科学院林木果树研究所,上海市设施园艺技术重点实验室,上海 201106)
  • 收稿日期:2009-12-03 修回日期:2010-03-01 出版日期:2010-03-25 发布日期:2010-03-25

YE Zheng-wen;DU Ji-hong;SU Ming-shen;LI Liu-lin;ZHANG Shao-ling   

  • Received:2009-12-03 Revised:2010-03-01 Online:2010-03-25 Published:2010-03-25

摘要: 在人工气候培养箱内对‘锦绣’黄桃(Prunus persica‘Jinxiu’)花器官进行高温处理,利
用光学显微镜探讨了高温对小孢子形成和花粉发育的影响。结果表明:与对照相比,35 ℃高温处理1 h 对花粉量影响不显著,随着处理时间增加,花粉量、萌发率以及花粉管生长量显著下降,5 h 高温处理的花粉量仅为对照的1.9%,且基本不萌发;5 h 高温处理部分花粉母细胞减数分裂后不能形成正常的四分体,而形成二分体、三分体以及多分体。随着花药胼胝质的解体,花药内出现了多核小孢子。高温处理后小孢子细胞质收缩解体,随后小孢子壁也解体;高温处理的花药壁细胞染色浅,绒毡层提前解体,说明绒毡层不能继续为小孢子发育提供营养物质,使小孢子发育受阻,造成小孢子逐渐消失。

关键词: 桃, 高温, 小孢子, 花粉发育

Abstract: Effects of high temperature on microsporogenesis and pollen development of ‘Jinxiu’yellow peach was studied in artificial climate chamber. The results showed that: There was no significant difference between pollen quantity in anthers treated under 35 ℃ for an hour and that under control(20 ℃). With increasing duration of high temperature(35 ℃)treatment, quantity, germination rate and length of pollen tubes significantly decreased. While the duration reached to 5 hours, pollen quantity was as only 1.9 % of the control, also showed nearly no bourgeon. Some pollen mother cells under 5 hours of 35 ℃ can not generate normal tetrad,but form dyad,triad and polyad,further,multinuclear microspores appeared along with callose degradation. After high temperature treatment,cytoplasm of microspores contracted and degenerated,also followed with microspores wall degradation. As regards cells of anther wall they were dyed light,due to the early disintegrated of tapetum cells. It was suggested that disappearance of microspore be induced by the disability of nutrition supply from tapetum cells.

Key words: peach, high temperature, microspore, pollen development

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