促生菌NK1诱导白菜幼苗对炭疽病抗性的研究

园艺学报 ›› 2008, Vol. 35 ›› Issue (4): 595-598.

促生菌NK1诱导白菜幼苗对炭疽病抗性的研究

梁建根;吴吉安;竺利红;桑金隆;姚杭丽;施跃峰*

(浙江省农业科学院植物保护与微生物研究所，杭州310021)

收稿日期:2007-10-08 修回日期:2008-01-24 出版日期:2008-04-25 发布日期:2008-04-25
通讯作者: 施跃峰

Studies on the Resistance to Cabbage Anthracnose Induced by Plant Growth-Promoting Rhizobacteria NK1

LIANG Jian-gen，WU Ji-an，ZHU Li-hong，SANG Jin-long，YAO Hang-li，and SHI Yue-feng*

(Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou，310021, China)

Received:2007-10-08 Revised:2008-01-24 Online:2008-04-25 Published:2008-04-25
Contact: SHI Yue-feng

摘要/Abstract

摘要：

以植物根围促生细菌NK1菌株为诱导因子，对其诱导白菜抗炭疽病进行了研究。结果表明：用菌株NK1灌根处理白菜幼苗后，叶片上病斑数及病斑面积较对照明显减少（P<0.05），对炭疽病防效达68%。不同诱导方法诱抗效果呈显著性差异（P<0.05），其中以种子处理+灌根处理方法的诱导效果最好。对菌株NK1诱导持效期的研究发现，诱抗效果以菌株NK1处理白菜植株后的第5 d接种白菜炭疽病菌时最好。此外，用菌株NK1灌根法处理白菜根部后，白菜根部与叶片组织中的几丁质酶和β-1,3-葡聚糖酶的活性较对照都明显升高（P<0.05），表明菌株NK1能诱发白菜对炭疽病产生系统抗性。

关键词: 白菜, 植物根围促生细菌, 炭疽病, 诱导抗性

Abstract: The resistance to cabbage anthracnose induced by plant growth-promoting rhizobacteria strain NK1 was studied. The results indicated that the number of lesion and its area on the leaves were both significantly reduced compared with the control (P <0.05), the induced effectiveness to cabbage anthracnose reached 64% after strain NK1 treatment. Meanwhile, the induced effectiveness was significantly different by using different methods (P <0.05), that of seed treatment and root irrigation was the best. In addition, the research found that the induced effectiveness was the best under the condition of treating the cabbage seedlings with NK1 before inoculating Colletotrichum higginsianum as early as five days. Compared with the control, chitinase and β-1, 3-glucanase activity of the cabbage leaves and roots significantly increased after NK1 treatment(P <0.05), which suggested that strain NK1 could systemicly induce cabbage to produce resistance to cabbage anthracnose.

Key words: cabbage, plant growth-promoting rhizobacteria, anthracnose, induced resistance

中图分类号:

S642.2
S476

梁建根;吴吉安;竺利红;桑金隆;姚杭丽;施跃峰. 促生菌NK1诱导白菜幼苗对炭疽病抗性的研究[J]. 园艺学报, 2008, 35(4): 595-598.

LIANG Jian-gen;WU Ji-an;ZHU Li-hong;SANG Jin-long;YAO Hang-li;and SHI Yue-feng. Studies on the Resistance to Cabbage Anthracnose Induced by Plant Growth-Promoting Rhizobacteria NK1[J]. ACTA HORTICULTURAE SINICA, 2008, 35(4): 595-598.

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