苹果属观赏海棠实生单株亲本的AFLP鉴定

园艺学报 ›› 2010, Vol. 37 ›› Issue (1): 121-128.

苹果属观赏海棠实生单株亲本的AFLP鉴定

赵天田;沈红香;姚允聪3;曹庆芹;宋婷婷

(北京农学院植物科学技术学院, 北京102206)

收稿日期:2009-05-05 修回日期:2009-11-16 出版日期:2010-01-25 发布日期:2010-01-25
通讯作者: 姚允聪

Identification of Parentage of Ornamental Crabapple Seedlings Using AFLPMarkers

ZHAO Tian-tian;SHEN Hong-xiang; YAO Yun-cong*;CAO Qing-qin;SONG Ting-ting

( Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China)

Received:2009-05-05 Revised:2009-11-16 Online:2010-01-25 Published:2010-01-25
Contact: YAO Yun-cong

摘要/Abstract

摘要： 应用AFLP技术, 对以苹果属观赏海棠品种王族(Malus ‘Royalty’) 为母本, 以5个材料为父本的38个实生单株进行多态性分析。结果表明: 筛选出的9对分辨率高的引物共扩增出349条带, 其中多态性带303条, 多态位点百分率达86.8%。根据聚类分析和电泳图谱扩增得到亲本材料的特征带, 鉴定出19株实生单株的父本可能为‘宝石’ (Malus‘Jewelberry’) ; 7株实生单株的父本可能为海棠花(Malus spectabilis Borkh. ) ; 3株实生单株的父本可能为草莓果冻(Malus‘Strawberry Parfait’) ; 不能确定其父本的实生单株有9株。

关键词: 苹果属, 观赏海棠, 实生单株, AFLP, 亲本鉴定

Abstract: The polymorphism was analyzed by AFLP in 38 Ornamental Crabapple seedlings from female parent, Malus‘Royalty’, and 5 possible male parents. Results showed that the total 349 informative AFLP fragments were generated using the selected 9 EcoR Ⅰ - Mse Ⅰ p rimer combinations with high resolution capacity. Among them there were 303 polymorphic fragments with a polymorphism percentage of 86.8%. According to the characteristic bands of electrophoretogram and Cluster Analysis, the male parents of 19, 7 and 3 seedlings were identified to be Malus‘Jewelberry’, Malus spectabilis Borkh. and Malus‘Strawberry Parfait’respectively. The remnant 9 seedlings could not be confirmed or identified.

Key words: Malus, Ornamental Crabapple, seedling, AFLP, identification of parentage

中图分类号:

S 685.99

赵天田;沈红香;姚允聪;曹庆芹;宋婷婷. 苹果属观赏海棠实生单株亲本的AFLP鉴定[J]. 园艺学报, 2010, 37(1): 121-128.

ZHAO Tian-tian;SHEN Hong-xiang;YAO Yun-cong;CAO Qing-qin;SONG Ting-ting. Identification of Parentage of Ornamental Crabapple Seedlings Using AFLPMarkers[J]. ACTA HORTICULTURAE SINICA, 2010, 37(1): 121-128.

[1]	孙思邈, 王昆, 高源, 王大江, 李连文. 观赏海棠新品种‘紫辰’[J]. 园艺学报, 2022, 49(S2): 267-268.
[2]	张萌, 单玉莹, 杨业波, 翟飞飞, 王兆山, 巨关升, 孙振元, 李振坚. 中国石斛属植物遗传资源的AFLP分析[J]. 园艺学报, 2022, 49(6): 1339-1350.
[3]	栾爱萍1，贺军虎1,*，刘佳柔2，张伟2，谢桃2，何业华2. 矮凤梨短营养期突变体‘14-1’分子变异的AFLP分析[J]. 园艺学报, 2020, 47(4): 734-740.
[4]	高源, 王大江, 王昆, 丛佩华, 李连文, 朴继成. 苹果属植物种质多样性的SLAF-seq分析[J]. 园艺学报, 2020, 47(10): 1869-1882.
[5]	张璐璐1，毛云飞1，张灿洪2，张多娇2，沈向1,*. 观赏海棠新品种‘多娇’[J]. 园艺学报, 2019, 46(S2): 2907-2908.
[6]	史锋厚，梁钰莹，沈永宝*. 观赏海棠新品种‘红霞’[J]. 园艺学报, 2019, 46(9): 1861-1862.
[7]	沙广利*，黄粤，马荣群，葛红娟，张蕊芬，孙吉禄，王芝云，宫象晖. 观赏海棠新品种‘大棠婷美’[J]. 园艺学报, 2019, 46(9): 1863-1864.
[8]	胡丁猛1，韩友吉1，许景伟1,*，王立辉2，朱升祥3，囤兴建1. 观赏海棠新品种‘昌辉’[J]. 园艺学报, 2018, 45(S2): 2811-2812.
[9]	胡丁猛1，韩友吉1，许景伟1,*，王立辉2，囤兴建1，朱升祥3. 晚花期观赏海棠新品种‘昌红’[J]. 园艺学报, 2018, 45(S2): 2813-2814.
[10]	王萱1,，刘晓静1,，邢世岩1,**，孔倩倩1，张艳辉1，孙立民1，高燕2. 中国部分古银杏资源遗传多样性的AFLP 分析及核心种质的构建[J]. 园艺学报, 2016, 43(2): 249-260.
[11]	申艳红*，陈晓静，蔡雪玲，叶一江，耿姣姣，杨菲颖. 番木瓜半胱氨酸蛋白酶基因CpCP的分离及表达分析[J]. 园艺学报, 2015, 42(9): 1789-1797.
[12]	杨树华1，郭宁1,2，刘海星1，葛红1,*. 新疆天山地区弯刺蔷薇居群遗传多样性AFLP分析[J]. 园艺学报, 2015, 42(9): 1823-1830.
[13]	吴岐奎, 邢世岩, 王萱, 孙立民. 核用银杏品种遗传关系的AFLP分析[J]. 园艺学报, 2015, 42(5): 961-968.
[14]	吕德国，杜国栋，秦嗣军，马怀宇，刘国成. 观赏海棠新品种‘凤凰’[J]. 园艺学报, 2015, 42(4): 805-806.
[15]	孙晓梅，杨盼盼，陆秀君，周文强，王丹，杨宏光. 芍药种子cDNA-AFLP体系的建立[J]. 园艺学报, 2015, 42(3): 576-584.