5 - 氨基乙酰丙酸对亚适宜温光条件下黄瓜幼苗光合特性的影响

园艺学报 ›› 2010, Vol. 37 ›› Issue (1): 65-71.

5 - 氨基乙酰丙酸对亚适宜温光条件下黄瓜幼苗光合特性的影响

刘玉梅2,3; 艾希珍2; 于贤昌1,2*

(¹中国农业科学院蔬菜花卉研究所, 北京100081; ² 山东农业大学园艺科学与工程学院作物生物学国家重点实验室,山东泰安271000; ³ 菏泽学院园林工程系, 山东菏泽274000)

收稿日期:2009-09-15 修回日期:2009-12-14 出版日期:2010-01-25 发布日期:2010-01-25
通讯作者: 于贤昌

Effects of ALA on Photosynthesis of Cucumber Seedlings under SuboptimalTemperature and Light Intensity

LIU Yu-mei2, 3 ;AI Xi-zhen2 ; YU Xian-chang1, 2*

(1 Institute of Vegetables and Flowers, Chinese Academ y of Agricultural Sciences, B eijing 100081, China; 2 College of Horticul2ture Science and Engineering, S tate Key Laboratery of Crop B iology, Shandong Agricultural University, Taipan, Shandong271018, China; 3 Departm ent of Landscape Engineering, Heze College, Heze, Shandong 274000, China)

Received:2009-09-15 Revised:2009-12-14 Online:2010-01-25 Published:2010-01-25
Contact: YU Xian-chang

摘要/Abstract

摘要： 为了弄清外源5 - 氨基乙酰丙酸(ALA) 提高亚适宜温光下黄瓜幼苗光合作用的效果, 以‘津优1号’黄瓜为试材, 用0.5 mg·L - 1 ALA喷洒幼苗, 利用光照培养箱模拟亚适宜温光条件(昼/夜温度18 ℃/12 ℃, 光量子通量密度300μmol·m - 2 ·s- 1 ) , 研究ALA对幼苗气体交换参数、叶绿素荧光参数及光合酶活性的影响。结果表明: 亚适宜温光下, 黄瓜幼苗叶片的净光合速率( Pn ) 和气孔导度(Gs ) 、羧化效率(CE) 、表观量子效率(AQY) 、光下实际光化学效(ΦPⅡ ) 、光化学猝灭系数( qP ) 、天线转化效率(Fv ′/Fm ′) 及核酮糖- 1, 5 - 二磷酸羧化酶(RuBPCase) 、果糖- 1, 6 - 二磷酸酶( FDPase) 活性等均明显降低, 胞间CO2浓度(Ci ) 升高, 光补偿点(LCP) 、CO2补偿点(CCP) 显著下降。与水处理相比, ALA处理的Pn、Gs、CE、AQY、ΦPSⅡ、qP、Fv ′/Fm ′及RuBPCase和FDPase活性等有不同程度提高,Ci、LCP和CCP有所降低。亚适宜温光下, 黄瓜幼苗光合速率下降的主要原因是非气孔限制, 外源ALA能明显提高其光合作用。

关键词: 关键词: 黄瓜, 5 - 氨基乙酰丙酸, 亚适宜温光, 光合作用中图分类号:

Abstract: To make clear the influence of ALA on cucumber photosynthesis under suboptimal temperature ( 18 ℃/12 ℃) and light intensity (300μmol·m- 2 ·s-1 ) , 015 mg·L - 1 ALA was applied by spraying, and the gas exchange parameters, chlorophyll fluorescence parameters, activities of RuBPCase andFDPase were investigated. The results showed that suboptimal temperature and light intensity decreased the net photosynthetic rate ( Pn ) , stomatal conductance (Gs ) , carboxylation efficiency (CE ) , apparent quantum yield (AQY) , actual photochemical efficiency of PSⅡ (ΦPSⅡ ) , photochemical quenching ( qP ) , antenna transformation efficiency ( Fv ′/Fm ′) , and activities of RuBPCase and FDPase of cucumber seedlings, but increased the intercellular CO2 concentration (Ci ). Furthermore, compared with the normal temperature and light intensity, suboptimal temperature and light intensity led to significant decrease in the light compensation point (LCP) and CO2 compensation point (CCP). while application of 015 mg·L - 1 ALA increased the Pn.Gs , CE, AQY, ΦPSⅡ , qP , Fv ′/Fm ′, and activities of RuBPCase and FDPase, decreased the Ci , LCP and CCP of cucumber seedlings under suboptimal temperature and light intensity. These data suggested that nonstomatal limitation was the main cause of the decrease of Pn , ALA p layed a positive role in alleviating the effect of suboptimal temperature and light intensity on photosynthesis of cucumber seedlings.

Key words: Key words: cucumber, 52aminolevulinic acid (ALA ), subop timal temperature and light intensity, photosynthesis

中图分类号:

S 626

刘玉梅;艾希珍;于贤昌;. 5 - 氨基乙酰丙酸对亚适宜温光条件下黄瓜幼苗光合特性的影响[J]. 园艺学报, 2010, 37(1): 65-71.

LIU Yu-mei;;AI Xi-zhen;YU Xian-chang;. Effects of ALA on Photosynthesis of Cucumber Seedlings under SuboptimalTemperature and Light Intensity[J]. ACTA HORTICULTURAE SINICA, 2010, 37(1): 65-71.

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