关键词: 百合, 远缘杂交, 胚胎发育

Abstract: This experiment was based on paraffin methods to observe ovule of different development stages in hybrid combination between Lilium asiatic hybrids ‘Navona’ × Lilium pumilum, while the female parent was Asiatic hybrids and male parent is wild type. We found that fertilization and embryonic
development process of this distant hybrid combination were complicated, there were four conditions during the development. Fertilization could not be completed and embryonic sac member cells would disintegrate at last, which was one of the conditions. The second one was only the egg cell be fertilized, embryo will be obtained without endosperm. The third one was just the opposite, only polar nuclear cells were fertilized and endosperm be obtained but embryo. The last condition was double fertilization completed, both embryo and endosperm were obtained. Development of hybrid idiosome in Lilium went through stages of zygote, proembryo, spherical embryo, pear-shaped like embryo and clavate embryo. Before the clavate embryo stage,
nutrition which supporting the embryo development was mainly absorbed from nucellus and integument bythe suspensor. When the embryo began to differentiate within the body, its shape was rod-like, suspensor disintegration. After that, embryo’s nutritional requirements were mainly provided by the endosperm cells. Development of Lilium endosperm was belonged to nuclear type. One of the reasons which probably inducing
hybrid embryonic abortion was no nurture do embryo gain from endosperm, and this kind of embryo would grow not better than the stage of spherical embryo, another probable reason was endosperm growing slowly
or even stop growing, which resulting in embryonic abortion at last. Forty-five days after pollination was the best time to carry out embryo rescue in this hybrid combination.

Key words: Lilium, distant hybridization, embryonic development