马铃薯广谱抗病Rpi-blb1 同源基因抗性被进化晚疫病菌株克服

园艺学报 ›› 2010, Vol. 37 ›› Issue (2): 241-246.

马铃薯广谱抗病Rpi-blb1 同源基因抗性被进化晚疫病菌株克服

朱素贤1,3;祝军1,*;李颖2;Nijenhuis Maarten 3;Bergervoet Marjan 3;Rietman Hendrik 3;Jacobsen Evert 3

(¹青岛农业大学园林园艺学院，山东青岛 266109；² 中国农业科学院蔬菜花卉研究所，北京 100081；³ 瓦赫宁根大学与研究中心国际植物研究所，瓦赫宁根 6700AJ)

收稿日期:2009-05-27 修回日期:2009-12-02 出版日期:2010-02-25 发布日期:2010-02-25

Broad Spectrum Resistance from Rpi-blb1 Homologous R-genes Has BeenBroken by Phytophthora infestans Isolates Collected from Solanumstoloniferum

ZHU Su-xian1, 3;ZHU Jun1,*;LI Ying2;Nijenhuis Maarten3;Bergervoet Marjan3;Rietman Hendrik3;Jacobsen Evert3

(¹College of the Horticulture and Landscape，Qingdao Agricultural University, Qingdao，Shandong 266109，China；²Institute of Vegetable and Flowers，Chinese Academy of Agricultural Sciences, Beijing 100081，China； ³Plant Research International， Wageningen University and Research Centre，Wageningen 6700 AJ，The Netherlands)

Received:2009-05-27 Revised:2009-12-02 Online:2010-02-25 Published:2010-02-25

摘要/Abstract

摘要： 马铃薯晚疫病广谱抗性基因Rpi-blb1（克隆于Solanum bulbocastanum）及其两个同源抗病基因Rpi-sto1（克隆于Solanum stoloniferum）和Rpi-pta1（克隆于Solanum papita）的抗性可能已被高度进化的晚疫病菌株所克服。为证实此观点，将从荷兰田间和墨西哥野外S. bulbocastanum 和S. stoloniferum 病株上采集的晚疫病菌株重新接种于具有共同遗传背景（感病栽培品种Desiree）的Rpi-blb1 及其同源基因转化体和3个野生种植株上，测试Rpi-blb1 及其同源基因的抗性是否已被克服。结果表明，采自墨西哥野外S. stoloniferum的两个菌株Pic99189 和Pic99192 已经克服了Rpi-blb1 及其同源基因的抗性；在野生种植株的测试中，只有S. papita 的抗性明显地被菌株Pic99192 所克服。同时发现在转基因植株离体叶片接种试验中，高抗晚疫病菌的转化体需要在多个转化体间进行选择。从采自中国4 个不同地区晚疫病菌株接种sto1.Desiree 转化体的小规模测试中可推断，Rpi-blb1 及其同源抗性基因在中国仍具有潜在的应用价值。

关键词: 马铃薯, 晚疫病, 同源抗病基因

Abstract: The resistance of potato broad spectrum resistance gene Rpi-blb1（cloned from Solanum bulbocastanum）and its homologues Rpi-sto1（cloned from Solanum stoloniferum）and Rpi-pta1（cloned from Solanum papita）might be broken by advanced Phytophthora infestans isolates. Isolates potentially breaking this resistance could be found in Dutch potato fields on accessions of S. bulbocastanum and S. stoloniferum with these R-genes and in Mexico in nature on the same species. Here, these isolates have been retested on
transgenic Desiree containing Rpi-blb1, Rpi-sto1 or Rpi-pta1 and on accessions of all three species（blb8005, sto389-4 and pta767-1）which are harboring one of the three cloned R-genes. It turned out that two isolates
from Mexico, Pic99189 and Pic99192, found on S. stoloniferum, have broken the resistance from Rpi-blb1, Rpi-sto1 and Rpi-pta1 in cv. Desiree background. In addition, it was observed that the accessions harboring
the same R-gene still were resistant to these isolates except Pic99192, which had clearly broken the S. papita accession. Another observation was that transgenic plants need be selected for strong expression of resistance
to P. infestans in detached leaf assay (DLA). A pilot experiment with a Rpi-sto1 containing Desiree transformant，inoculated with Chinese isolates originated from 4 regions, showed high level of resistance and its potential application in China.

Key words: potato, late blight, homologous resistance genes

朱素贤;祝军;李颖;Nijenhuis Maarten;Bergervoet Marjan;Rietman Hendrik;Jacobsen Evert . 马铃薯广谱抗病Rpi-blb1 同源基因抗性被进化晚疫病菌株克服[J]. 园艺学报, 2010, 37(2): 241-246.

ZHU Su-xian;;ZHU Jun;LI Ying;Nijenhuis Maarten;Bergervoet Marjan;Rietman Hendrik;Jacobsen Evert. Broad Spectrum Resistance from Rpi-blb1 Homologous R-genes Has BeenBroken by Phytophthora infestans Isolates Collected from Solanumstoloniferum[J]. ACTA HORTICULTURAE SINICA, 2010, 37(2): 241-246.

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