新铁炮百合单倍体植株的诱导

园艺学报 ›› 2010, Vol. 37 ›› Issue (2): 263-276.

新铁炮百合单倍体植株的诱导

韩秀丽;田晓明;贾桂霞^*

(北京林业大学园林学院，国家花卉工程技术研究中心，北京100083)

收稿日期:2009-03-26 修回日期:2010-01-21 出版日期:2010-02-25 发布日期:2010-02-25

Induction of Haploid Plantlets for Lilium × formolongi

HAN Xiu-li;TIAN Xiao-ming;JIA Gui-xia*

(College of Landscape Architecture, Beijing National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing 100083, China)

Received:2009-03-26 Revised:2010-01-21 Online:2010-02-25 Published:2010-02-25

摘要/Abstract

摘要： 在新铁炮百合‘雷山1 号’减数分裂和小孢子发育进程研究的基础上，确定了与小孢子不同发育时期相对应的花蕾长度和花药颜色等外部形态特征；筛选出‘雷山1 号’诱导愈伤组织适宜的培养基：基本培养基为MS 改良培养基，添加2,4-D 0.5 mg · L^-1、KT 4.0 mg · L^-1，蔗糖浓度30 ~ 90 g · L^-1。对不同发育阶段花药诱导培养的结果显示：最佳诱导时期为小孢子发育的早、中期，即花蕾长度为24 ~ 25 mm 时，诱导率达44%。将花药愈伤组织转移至MS 添加2.0 mg · L-1NAA，可诱导分化形成花粉植株，分化率达100％，其中单倍体达43%。

关键词: 新铁炮百合, 小孢子, 单倍体

Abstract: Abstract: Based on the meiosis and microspore development of Lilium × formolongi ‘Raizan 1’, the length of flower bud and the colors of anther relative to the development stages were determined. The suitable media was selected for haploid callus induction of the ‘Raizan 1’, that was improved MS supplemented with 2,4-dichlunruphenoxyacetic（2,4-D）0.5 mg · L^-1 and kinetin（KT）4.0 mg · L^-1. The results showed the effects of sucrose concentration in the 30–90 g · L^-1 were not significant on the microspore callus induction. Microspores at early and mid-stage were more suitable for culture. Induction percentage of haploid calli were 44%, as bud length were 24–25 mm. Plantlet was obtained when haploid calli was transferred to the MS medium supplemented with 2.0 mg · L^-1 naphthaleneacctic acid（NAA）. The induction rate of plantlets were 100%，there into，plantlets of haploid were 43%.

Key words: Lilium × formolongi, microspore, haploid

韩秀丽;田晓明;贾桂霞. 新铁炮百合单倍体植株的诱导[J]. 园艺学报, 2010, 37(2): 263-276.

HAN Xiu-li;TIAN Xiao-ming;JIA Gui-xia. Induction of Haploid Plantlets for Lilium × formolongi[J]. ACTA HORTICULTURAE SINICA, 2010, 37(2): 263-276.

[1]	吕红豪, 杨丽梅, 方智远, 张扬勇, 庄木, 刘玉梅, 王勇, 季家磊, 李占省, 韩风庆. 春甘蓝新品种‘中甘27’和‘中甘28’[J]. 园艺学报, 2022, 49(S1): 63-64.
[2]	李智军, 曾晶, 谭铭喜, 谢景. 芥蓝游离小孢子胚胎发生途径及2,4-D对成胚的影响[J]. 园艺学报, 2021, 48(8): 1605-1618.
[3]	党江波, 党江波, 韩国辉, 李彩, 向素琼, 郭启高, 梁国鲁. 柑橘倍性操作技术与育种现状、难点及展望[J]. 园艺学报, 2021, 48(4): 791-810.
[4]	周霞, 邓英, 陈劲枫, . 瓜类作物离体雌核诱导单倍体研究进展[J]. 园艺学报, 2020, 47(9): 1810-1826.
[5]	李菲1,2，张淑江1，章时蕃1，武剑1，王晓武1，张慧1，孙日飞1,*，张振贤2. 基因枪法介导大白菜小孢子转基因技术研究初报[J]. 园艺学报, 2017, 44(1): 62-68.
[6]	张恩慧*，许忠民，程永安，杨安平，马青山. 甘蓝双单倍体系配制的早熟新品种‘秦甘62’[J]. 园艺学报, 2016, 43(S2): 2729-2730.
[7]	耿倩倩，王彦华，轩淑欣，毛清云，赵建军，申书兴. 添加甘蓝2 号染色体片段的大白菜易位系的获得及其遗传稳定性分析[J]. 园艺学报, 2016, 43(2): 261-270.
[8]	姚秋菊，张晓伟*，蒋武生，原玉香，王志勇，魏小春，赵艳艳，张强. 甘蓝新品种‘豫甘1号’[J]. 园艺学报, 2015, 42(S2): 2887-2888.
[9]	孙继峰，方智远，袁素霞，李占省，杨丽梅，庄木，张扬勇，孙培田，刘玉梅. 不同温度预处理对青花菜小孢子胚胎发生的影响[J]. 园艺学报, 2015, 42(3): 563-568.
[10]	王烨，顾兴芳*，张圣平，苗晗. 黄瓜未受精胚珠离体培养及单倍体植株再生[J]. 园艺学报, 2015, 42(11): 2174-2182.
[11]	胡永霞, 李晓峰, 轩淑欣, 申书兴, 王彦华. 利用小孢子培养创建大白菜—结球甘蓝易位系[J]. 园艺学报, 2014, 41(7): 1361-1368.
[12]	顾宏辉1,*，虞慧芳1，许映君2，陈纪算3，汪炳良4，王建升1，毛培成3，赵振卿1，盛小光1，王巍3. 青花菜新品种‘海绿’[J]. 园艺学报, 2014, 41(2): 391-392.
[13]	刘颖颖，刘世琦*，薛小艳，刘景凯，陈祥伟，冯磊. 大蒜未受精子房离体诱导单倍体的研究[J]. 园艺学报, 2013, 40(6): 1178-.
[14]	刘富强, 代双艳, 王彦华*?, 张成合, 申书兴. 单倍体大白菜减数分裂分析及单体植株的获得[J]. 园艺学报, 2013, 40(4): 767-.
[15]	顾宏辉, 赵振卿, 盛小光, 虞慧芳, 王建升, 孙利祥, 颜韶兵, 邹宜静. 松散型花椰菜新品种‘浙 091’[J]. 园艺学报, 2013, 40(12): 2537-2538.