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园艺学报 ›› 2003, Vol. 30 ›› Issue (02): 224-226.

• 研究报告 • 上一篇    下一篇

菜薹茎尖培养中的激素与热激调控

廖飞雄1;潘瑞炽2;何晓明3
  

  1. (1 广东省农业科学院花卉研究所,广州510640;2华南师范大学生命科学学院,广州510631;3广东省农业科学院蔬菜研究所,广州510640)
  • 收稿日期:2002-06-03 修回日期:2002-10-21 出版日期:2003-04-25 发布日期:2003-04-25

Effect of Plant Growth Regulators and Heat Shock on Shoot-tip Culture inBrassica campestris L.ssp. chinensis var. utilis Tsen et Lee.

Liao Feixiong1; Pan Ruichi2;He Xiaoming3
  

  1. (1 Floriculture Research Institute,Guangdong Academy Agriculture Science,Guangzhou 510640,China;2College of Life Science,South China Normal University,Guangzhou 510631,China;3 Vegetable Institute,Guangzhou Academy of Agricultura Science,Guangzhou 510640,China)
  • Received:2002-06-03 Revised:2002-10-21 Online:2003-04-25 Published:2003-04-25

摘要: BA和NAA不同浓度组合对菜薹茎尖培养会产生不同的影响,BA 0.8 mg·L-1 以上浓度能获得较好的增殖效果,NAA浓度不宜超过0.5 mg·L-1。KT和ZT混用不及单一使用效果好。外植体放置方式会影响茎尖的增殖效率。45 ℃ 2 h以内热激处理可促进菜薹茎尖的增殖。

关键词: 菜薹, 组织培养, 生长调节剂, 热激

Abstract: The propagation in vitro of shoot-tips and quality of plandets of Brassica campestris L.ssp. chinensis var. utilis Tsen et Lee.were affected by the combination of BA and NAA with diferent concentrations.Higher rate of shoot proliferation with better quality of planflets could be obtained when the concentration of BA in medium was mole than 0.8 mg·L-1 and optimal concentration of NAA was less than 0.5 mg·L-1.The efect of KT or ZT respectively on propagation WaS better than that of the combination of the both when total concentrations were identica1.The orientations of plant was found to afect the proliferation rate.Heat shock with shoot—tips at
45 ℃ for 2 h could stimulate the proliferation of shoots.

Key words: Brassica campestris L.ssp. chinens/s var. utilis Tsen et Lee., Tissue culture, Plant growthred t op3, Heat shock

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