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园艺学报 ›› 2004, Vol. 31 ›› Issue (4): 467-471.

• 研究论文 • 上一篇    下一篇

白菜和芜菁Ogura型雄性不育系与保持系的获得及其细胞学观察

崔辉梅 曹家树 张明龙 姚祥坦 向   

  1. ( 浙江大学蔬菜研究所,杭州310029; 石河子大学园艺系,石河子832003;殉浙江大学核农业科学研究所.杭州310029)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-08-25 发布日期:2004-08-25

Production of the Ogura Cytoplasmic Male Sterile(CMS)Lines of ChineseCabbage-pak-choi(Brassica campestris L.ssp.chinensis var.communis)and Turnip(B.campestris L.ssp.rapifera)and Cytological Observation ofTheir Sterile Organs

Cui Huimei,Cao Jiashu,Zhang Minglong,Yao Xiangtan .and Xiang Xun
  

  1. (。Institute of Vegetable Science,Zhefiang University,Hangzhou 3 1 0029,China: Department of Horticulture.Shihezi Unil)ersity.Shihezi 832003,China; Institute of Nuclear Agricultural Sciences,Zhejiang University,Hangzhou 3 1 0029,ChinⅡ)
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-08-25 Published:2004-08-25

摘要: 通过甘蓝型油菜Ogura细胞质雄性不育材料与白菜、芜菁的种问杂交和连续回交,获得了不
育性稳定的白菜、芜菁Ogura细胞质雄性不育系‘新选Ogu284’、‘矮脚黄Ogu33.14’和‘耐病 “18_4’
及其相应的保持系。细胞形态学研究表明:虽然这3个不育系核背景不同,但花药败育时朗基本相同.
均具有单核败育型的特征;小孢子到单核期后不再向前继续发育,而是迅速退化以至解体,丁F花前彻底
败育;不育系的花药在减数分裂早期绒毡层细胞中已出现液泡.有肥大趋势,而正常花药绒毡 的发育在
减数分裂过程中达到高峰,四分体时出现退化迹象,开花前完全解体。不同的Ogura细胞质雄性 育系,
虽然核背景不同,但花药败育的时期基本相同,说明Og.ra CMS胞质互作关系稳定,受核背景影响小

关键词: 白菜, 芜菁, Ogura细胞质雄性不育, 小孢子发生, 细胞形态学

Abstract: The Ogura CMS lines of Chinese cabbage—pak.choi f Br~sica campestris L.ssp.chinensis
Makino var.cornmunis Tsen et Lee.)and turnip[B.campestris L.ssp.rap r(z(Metzg.)Sinsk]were ob.
tained through interspeeifie hybridization between Ogura CMS in B. nap us and Chinese cabbage.pak.choi and
turnip,and successive backcrosses with Chinese cabbage—pak—choi and turnip as male parents. namely ‘Xin.
xuan Ogu28-4’, ‘Aijiaohuang Ogu33—14’, ‘Naibing Ogu18-4’.Cytomorphological studies indicated that
the inhibition of Ogura CMS B.campestris anther development may occur at the tetrad.uninucleic pollen stage.
The microspores stop developing at the uninucleic stage, quickly degenerated to disappear, and abort
absolutely before blossom. Tapetum of the male sterile line began vascuolation during meiosis and did n0t
disappear even when the uninucleic microspores had degenerated while that of the male fenile line began to
generate at tetrad stage and disappear after the pollens had matured. The different nuclear background 0f
Ogura CMS lines had similar abortive character.It might indicate that the relation of nueleo.cvt0plasmic inter
action iS steady,and the cytoplasmic effects were hardly affected by the nucleus of the sterile】ines.

Key words: Brassica campestris ssp.chinensis, Brassica campestris ssp. rap r(z, Brassifa rap0:Chi—nese cabbage’pak—choi, Turnip, Ogura CMS, Microsporogenesis, Cytomorphology