荔枝果实内切- 1, 4 - β - 葡聚糖酶基因( EG)的克隆及其表达分析

园艺学报 ›› 2009, Vol. 36 ›› Issue (12): 1733-1740.

荔枝果实内切- 1, 4 - β - 葡聚糖酶基因( EG)的克隆及其表达分析

吴富旺¹; 邝健飞²; 陆旺金¹ ;刘洪见³;陈建业^1*

(¹ 华南农业大学园艺学院, 广东省果蔬保鲜重点实验室, 广州510642; ² 中国科学院华南植物园, 广州510650;³浙江省亚热带作物研究所, 浙江温州325005)

收稿日期:2009-08-23 修回日期:2009-11-26 出版日期:2009-12-25 发布日期:2009-12-25
通讯作者: 陈建业

Cloning and Expression Analysis of EG Genes in Litchi Frui

WU Fu-wang¹; KUANG J ian-fei² ;LU Wang-jin¹;L IU Hong-jian³ ;and CHEN J ian-ye^1*

(¹Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University,Guangzhou 510642, China; ²South China B otanical Garden, Chinese Academ y of Sciences, Guangzhou 510650, China; ³ Institute of Subtropical Crops of Zhejiang Province, Wenzhou,Zhejiang 325005, China)

Received:2009-08-23 Revised:2009-11-26 Online:2009-12-25 Published:2009-12-25
Contact: CHEN Jian-ye

摘要/Abstract

摘要： 采用RT2PCR和RACE相结合的方法, 从荔枝果实中分离得到两个内切- 1, 4 -β - 葡聚糖酶基因( EG) 全长序列, 分别命名为LcEG1和LcEG2。推导的LcEG1和LcEG2蛋白均含有4个半胱氨酸Cys残基和两个保守的糖基水解酶启动位点。Northern blotting分析表明, LcEG1 mRNA在果皮发育过程中逐渐减弱, 而在果肉发育过程中逐渐增强; LcEG2仅在果肉发育的前期表达, 在果皮中检测不到其表达。LcEG1在荔枝易裂果品种‘糯米糍’果皮和果肉中的表达均明显强于在不易裂果品种‘淮枝’果皮和果肉中的表达。上述结果表明, LcEG1的表达可能参与荔枝果实发育, 并且与裂果密切相关, 而LcEG2可能只参与果肉的早期生长。

关键词: 荔枝;内切- 1;4 -&beta, - 葡聚糖酶;果实生长;裂果;基因;克隆;表达

Abstract: Abstract: Two different full lengths of EG cDNAs, termedas LcEG1 and LcEG2, were isolated from litchi fruit using RT-PCR and RACE (rapid amplification of cDNA ends) methods. Sequence alignment showed that four typical Cys residues and two predicted glycosyl hydrolase activesites were observed in the deduced LcEG1 and LcEG2 proteins.Northern blotting analysis showed that the level of LcEG1 mRNA accumulation gradually decreased in pericarpbut steadily increased in aril accompanying with fruit growth and development, while the expression of LcEG2 could only be detected in aril at early stage of fruit development.More importantly, the accumulation of LcEG1 mRNA was higher in cracking-susceptible cultivar Nuomici than that
in cracking-resistant cultivar Huaizhi at any stage of fruit development.Thus, it could be speculated that LcEG1 was closely related to the growth and cracking of litchi fruit, while, LcEG2 was only related to the growth of fruit at the early phase.
Key words: litchi; EG; fruit growth; fruit cracking; gene; clone; expression

吴富旺;邝健飞;陆旺金;刘洪见;陈建业. 荔枝果实内切- 1, 4 - β - 葡聚糖酶基因( EG)的克隆及其表达分析[J]. 园艺学报, 2009, 36(12): 1733-1740.

WU Fu-wang;KUANG J ian-fei;LU Wang-jin;L IU Hong-jian;and CHEN J ian-ye. Cloning and Expression Analysis of EG Genes in Litchi Frui[J]. ACTA HORTICULTURAE SINICA, 2009, 36(12): 1733-1740.