关键词: 结缕草, 胚性愈伤, 组织培养, 植株再生

Abstract: Mature caxyopses of two materials of Zoysia japonica Stend．coilected in diferent habitats were used as initial explants for callus induction. MS containing 2.0 mg/L 2,4-D induced calli at the relatively higher percentage of 31.2％-42.1％. Inclusion of 6-BA in callus induction med ium with various levels of 2,4-D made an important role for non-embryogenic calli changing into embryogenic calli．The embryogenic calli of higher percentage were induced by a combination of 2.0 mg／L 2,4-D and 0.1 mg/L 6-BA．The rates of shoot regeneration and rooting were different in different regeneration medium. The rates of shot regeneration and rooting were 46.8％-48.1％ at the level of 0.1 mg/L 2,4-D.

Key words: Zoysia japonica Steud., Embyrogenic callus, Tissue culture, Plant regeneration