秋水仙素处理‘罗田甜柿’获得12倍体再生植株

园艺学报 ›› 2003, Vol. 30 ›› Issue (03): 325-327.

秋水仙素处理‘罗田甜柿’获得12倍体再生植株

谷晓峰¹;罗正荣²

(¹华中农业大学园艺林学学院，武汉 430070; ²湖北民族学院园艺系，恩施 445000)

收稿日期:2002-06-05 修回日期:2002-09-19 出版日期:2003-06-25 发布日期:2003-06-25

Regeneration of Dodecapioid Plants from in Vitro Leave of 'Luotian Tianshi' Persimmon Treated with Colchicine

Gu Xiaofeng¹;Luo Zhengrong²

( ¹College of Horticulture and Forestry，Huazhong Agriculture University,Wuhan 430070, China; ²Department of Horticulture Hubei Institute for Nationalities，Enshi 445000，China)

Received:2002-06-05 Revised:2002-09-19 Online:2003-06-25 Published:2003-06-25

摘要/Abstract

摘要： 以‘罗田甜柿’试管苗离体叶片为材料，研究了秋水仙素浓度及处理时间对诱导染色体倍性变异的影响。结果表明：0.3％秋水仙素处理4～6 d，可诱导染色体加倍; 与对照比较, 再生变异植株的气孔孔径增大，密度降低，根尖染色体数2n=12x=180，叶片DNA含量为对照的两倍。LI、LⅡ、LⅢ细胞层均加倍的证据表明，获得的倍性变异株为同质12倍体。

关键词: 柿, 秋水仙素, 多倍体, 再生植株

Abstract: A study of inducing polyploidy by treating ‘Luotian Tianshi’persimmon in vitro leaflet explants with colchicine solution was perfonned．The results indieated that mutated planflets can be induced by treating the leaflet in 0.3％ colchicines solution for 4—6 d．Stomata of the mutated planflets were significantly larger than those of the normal planflets．but the density of stomata were reduced signmcantly．The chromosome number of root—tip cell of mutated plantlets was 2n=12x=180， and nuclear DNA content of the mutated planflets were twice as much as normal hexaploid plantlets．These results suggest that the ploidy of mutated planflets was autododecaploid．

Key words: Persimmon, Colchicine, Dodecaploid, Regeneration

中图分类号:

S 665.2

谷晓峰;罗正荣. 秋水仙素处理‘罗田甜柿’获得12倍体再生植株[J]. 园艺学报, 2003, 30(03): 325-327.

Gu Xiaofeng;Luo Zhengrong. Regeneration of Dodecapioid Plants from in Vitro Leave of 'Luotian Tianshi' Persimmon Treated with Colchicine[J]. ACTA HORTICULTURAE SINICA, 2003, 30(03): 325-327.

[1]	朱炜, 曹津津, 陈曦, 张葳, 孙榕泽, 朱绍才, 赵家庚, 崔雅琦, 王宇暄, 于晓南. 芍药新品种‘粉罗裙’‘千重粉黛’‘笑春风’和‘碧叶粉荷’[J]. 园艺学报, 2023, 50(2): 457-458.
[2]	蒋靖东, 韦壮敏, 王楠, 朱晨桥, 叶俊丽, 谢宗周, 邓秀新, 柴利军. 山金柑四倍体资源的发掘与鉴定[J]. 园艺学报, 2023, 50(1): 27-35.
[3]	张悦, 索玉静, 孙鹏, 韩卫娟, 刁松锋, 李华威, 张嘉嘉, 傅建敏, 李芳东. 柿种质资源果实形态多样性分析[J]. 园艺学报, 2022, 49(7): 1473-1490.
[4]	吴婷, 贾瑞冬, 杨树华, 赵鑫, 于晓南, 国圆, 葛红. 蝴蝶兰多倍体育种研究进展与展望[J]. 园艺学报, 2022, 49(2): 448-462.
[5]	杨思潮, 张萌, 张青林, 罗正荣. 中国甜柿自然脱涩特点及其机理研究进展[J]. 园艺学报, 2022, 49(12): 2659-2668.
[6]	周霞 1，张璐 1，周俊国 2，邓英 3，王怡 1，娄群峰 1，陈劲枫 1,*. 黄瓜未授粉子房离体培养获得胚囊再生植株[J]. 园艺学报, 2020, 47(3): 455-466.
[7]	周锐, 解凯东, 王伟, 彭珺, 谢善鹏, 胡益波, 伍小萌, 郭文武, . 依据多倍体形态特征快速高效发掘柑橘四倍体[J]. 园艺学报, 2020, 47(12): 2451-2458.
[8]	党江波1，梁国鲁1，李彩2，吴頔1，郭启高1,*，梁森林1，王鹏1. 果树多倍体砧木的研究现状与展望[J]. 园艺学报, 2019, 46(9): 1701-1710.
[9]	王春夏，尹玥，王志平，严瑞，付麟岚，孙红梅*. 多倍化兰州百合和细叶百合组培苗再生和耐非生物胁迫能力[J]. 园艺学报, 2019, 46(12): 2359-2368.
[10]	李华威1，王丽媛2，孙鹏2，索玉静2，韩卫娟2，买旖旎2，刁松锋2，袁德义1,，傅建敏2,. ‘龙岩野柿1号’雄花和两性花雌雄蕊发育的细胞形态学观察[J]. 园艺学报, 2019, 46(10): 1897-1906.
[11]	孙红梅1,2,*，付麟岚1，王志平1，盖美竹1，王春夏1,2. 基于体细胞胚发生的细叶百合和兰州百合多倍体诱导及鉴定[J]. 园艺学报, 2018, 45(6): 1136-1146.
[12]	刁松锋，韩卫娟，李芳东，孙鹏，索玉静，傅建敏*. 柿新品种‘中柿2号’[J]. 园艺学报, 2018, 45(5): 1009-1010.
[13]	王丽媛，孙鹏，张嘉嘉，傅建敏*，刁松锋，韩卫娟，索玉静，张悦. 柿野生雄性资源调查及其遗传多样性研究[J]. 园艺学报, 2018, 45(2): 261-278.
[14]	刁松锋1，李芳东1，梁臣2，孙鹏1，韩卫娟1，索玉静1，傅建敏1,*. 柿新品种‘中柿1号’[J]. 园艺学报, 2017, 44(s2): 2661-2662.
[15]	吕晔，胡兰，王利虎，刘平，刘孟军. 秋水仙素处理后‘无核丰’枣的减数分裂行为及2n花粉的形成[J]. 园艺学报, 2017, 44(1): 11-22.