建兰38个品种的RAPD分析

园艺学报 ›› 2008, Vol. 35 ›› Issue (2): 289-294.

建兰38个品种的RAPD分析

胡薇¹;黄儒珠^1*;潘晓华¹;李锦凤 ²;孙端³

( ¹福建师范大学生命科学学院，福州 350108;²中国兰协建兰样品园，福建上杭 364200; ³福建省林业检查总站，福州 350003)

收稿日期:2007-07-12 修回日期:2007-12-04 出版日期:2008-02-25 发布日期:2008-02-25
通讯作者: 黄儒珠

RAPD Analysis of Thirty-eight Cymbidium ensifolium Cultivars

HU Wei¹,HUANG Ru-zhu^1* ,PAN Xiao-hua¹,LI Jin-feng²,and SUN Duan³

( ¹College of Life Sciences, Fujian Normal University, Fuzhou 350108, China; ² Cymbidium ensifolium Garden, Chinese Cymbidium Association, shanghang, Fujian 364200, China;³ The General Inspection Station of Forestry of Fujian Province, Fuzhou 350003, China)

Received:2007-07-12 Revised:2007-12-04 Online:2008-02-25 Published:2008-02-25
Contact: HUANG Ru-zhu

摘要/Abstract

摘要：

应用RAPD标记对建兰38个品种的遗传多样性和亲缘关系进行分析。用筛选的18个10 bp随
机引物对其DNA进行PCR扩增，共扩增出116个位点，其中多态位点103个，多态位点比率占88.79%，表明建兰38个品种具有丰富的遗传多样性。38个品种间的遗传距离为0.0420~0.5385(均值0.2902)。基于RAPD标记的建兰38个品种的UPGMA聚类结果支持将建兰分为彩心和素心两个变种，以及素心多由彩心变异而来的传统分类观点。研究发现：引物S153-650 bp位点是'闽西鱼魫'、 '鱼魫大贡'、 '鱼魫'和'银边鱼魫'的特异标记，引物S38-1 200 bp位点是'十六罗汉'和'鱼魫'的特异标记，引物S38-800 bp位点缺失是'马耳四季'的特异标记。

关键词: 建兰, 品种, 遗传多样性, 聚类分析, RAPD

Abstract:

DNA from thirty-eight Cymbidium ensifolium cultivars was examined using polymerase chain reaction (PCR) to determine the efficiency of randomly amplified polymorphic DNA (RAPD) markers in genetic diversity and genetic relationship. A total of 116 RAPD markers, 88.79% of which were polymorphic, were produced from 18 10mer arbitrary primers. Genetic distances among the cultivars were estimated based on the amount of band sharing and ranged from 0.0420~0.5385 with an average of 0.2902. Unweighted pair-group method with arithmetic averaging (UPGMA) of genetic distances estimates grouped 'suxin' cultivars and 'caixin' cultivars together with each other, and 'suxin' cultivars diverged from 'caixin' cultivars, thereby agreeing with known traditional classification information. Four cultivars, 'Minxi Yuchen', 'Yuchen Dagong', 'Yuchen' and 'Yinbian Yuchen' could be distinguished from all the rest based only on the S153-650 fragment, and 'Shiliu Luohan' and 'Yuchen' based only on the S38-1 200 fragment. Cultivar 'Ma′er Siji' could be differentiated from all the rest by lack of the S38-800 fragment.

Key words: Cymbidium ensifolium, cultivar, genetic diversity, cluster analysis, RAPD

中图分类号:

S 682.31

胡薇;黄儒珠;潘晓华;李锦凤;孙端. 建兰38个品种的RAPD分析[J]. 园艺学报, 2008, 35(2): 289-294.

HU Wei;HUANG Ru-zhu;PAN Xiao-hua;LI Jin-feng;and SUN Duan. RAPD Analysis of Thirty-eight Cymbidium ensifolium Cultivars [J]. ACTA HORTICULTURAE SINICA, 2008, 35(2): 289-294.

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