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园艺学报 ›› 2003, Vol. 30 ›› Issue (5): 621-622.

• 研究报告 • 上一篇    下一篇

扎米莲( Zamioculcas zamiifolia) 叶片的植株再生

施和平;梁朋   

  1. (华南师范大学生命科学学院, 广州510631)
  • 收稿日期:2003-03-25 修回日期:2003-06-02 出版日期:2003-10-25 发布日期:2003-10-25

Plantlet Regeneration from Leaf Explants of Zamioculcas zamiifolia

Shi Heping;Liang Peng   

  1. ( School of Life Science , South China Normal University , Guangzhou 510631 , China)
  • Received:2003-03-25 Revised:2003-06-02 Online:2003-10-25 Published:2003-10-25

摘要: 建立了从扎米莲叶片直接再生植株的有效方法。结果表明, 扎米莲叶片外植体在仅加6-BA 或
NAA 或2 ,4-D 的培养基中培养8 周后都不能产生幼芽; 但在含不同浓度6-BA (1.0~2.0 mg/L) 和NAA(0.02~0.2 mg/L) 组合的MS 培养基上培养3 周后开始产生幼芽, 其幼芽分化的最佳培养基为MS + 6-BA 2.0 mg/L + NAA 0.02 mg/L。再生芽在添加IBA 0.5 mg/L 的MS 培养基中生根。

关键词: 扎米莲, 植株再生, 叶片

Abstract: An efficient regeneration protocol was developed through direct shoot regeneration from leaf explants of Zamioculcas zamiifolia Engl . The results showed no adventitious shoots were visible from leaf explants cultured
for 8 weeks on the mediumonly supplemented with 6-BA , NAA or 2 ,4-D. Adventitious shoot differentiation could occur directly from leaf explants cultured for 3 weeks on MS medium supplemented with 6-BA 1.0 - 2.0 mg/ L and NAA 0.02 - 0.2 mg/ L. MS medium supplemented with 6-BA 2.0 mg/ L and NAA 0.02 mg/ L was most effective. The regenerated shoots rooted in the MS medium with IBA (indole butyric acid) 0.5 mg/ L.

Key words: Zamioculcas zamiifolia Engl ., Plantlet regeneration, Leaves

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