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园艺学报 ›› 2003, Vol. 30 ›› Issue (5): 615-617.

• 研究报告 • 上一篇    下一篇

木兰科两个杂交组合的细胞学研究

龚洵1,2;张国莉1;潘跃芝1   

  1. (1 中国科学院昆明植物研究所, 昆明650204 ; 2 中山大学生命科学学院, 广州510275)
  • 收稿日期:2002-12-25 修回日期:2003-03-10 出版日期:2003-10-25 发布日期:2003-10-25

A Cytological Study on Two Hybridized Combinations of Magnoliaceae

Gong Xun1,2;Zhang Guoli1;Pan Yuezhi1   

  1. ( 1 Kunming Institute of Botany , Chinese Academy of Sciences , Kunming 650204 , China ; 2 School of Life Science , Zhongshan University , Guangzhou 510275 , China)
  • Received:2002-12-25 Revised:2003-03-10 Online:2003-10-25 Published:2003-10-25

摘要: 研究了木兰科中木兰属( Magnolia) 和含笑属( Michelia) 间两个杂交组合后代的染色体。杂交组合紫玉兰( ♀) ×云南含笑( ♂) 后代的染色体数目为2n = 3x = 57 , 正好是四倍体紫玉兰(2n = 4x =76) 和二倍体云南含笑(2n = 2x = 38) 的染色体半数之和, 证明该F1 为两者的杂交种; 杂交组合球花含笑( ♀) ×广玉兰( ♂) 后代的染色体数目为2n = 2x = 38 , 与二倍体球花含笑(2n = 2x = 38) 相同, 而不是球花含笑和广玉兰(2n = 6x = 114) 的染色体半数之和, 证明该F1 代不是真正的杂交种, 可能是无融合生殖的结果。本研究结果支持含笑属与木兰属玉兰亚属具有较近亲缘关系的观点。

关键词: 木兰属, 含笑属, 杂交组合, 染色体

Abstract: The chromosome number and size of two intergeneric hybridized combinations between Magnolia and Michelia in Magnoliaceae were studied in this paper. Magnolia liliflora is a tetraploid species with a chromosome number of 2n = 4x = 76 , Michelia yunnanensis is diploid species with a chromosome number of 2n = 2x = 38.The intergeneric hybridized combination , Magnolia liliflora ( ♀) ×Michelia yunnanensis ( ♂) , is triploid with a chromosome number of 2n = 3x = 57 , which was a half of the total chromosome number of its two parents. Of
which , 38 chromosomes from M. liliflora are smaller , and 19 chromosomes from M. yunnanensis are larger.So , the hybrid was confirmed to be crossbreeding filial generation of M. liliflora and M. yunnanensis. Michelia sphaerantha is a diploid species with a chromosome number of 2n = 2x = 38. Magnolia grandiflora is a hexaploid species with a chromosome number of 2n = 6x = 114. The intergeneric hybridized combination , M. sphaerantha ( ♀) ×M. grandiflora ( ♂) , is a diploid with a chromosome number of 2n = 2x = 38 , which is the same to that of its female parent , M. sphaerantha , in number and size. So , the hybridized combination is not a real hybrid , and the filial generation may be resulted from apomixes. The study showed that cytological method is one of efficient measures applied to early identification in crossbreeding of Magnoliaceae. The cross - compatibility indicated that Michelia is closely allied with Magnolia subgenus Yulania and is far allied with Magnolia subgenus Magnolia in phylogeny.

Key words: Magnolia , Michelia, Hybridized combination, Chromosome

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