番茄myb基因片段的克隆及在过敏性反应时的表达

园艺学报 ›› 2003, Vol. 30 ›› Issue (5): 589-591.

番茄myb基因片段的克隆及在过敏性反应时的表达

蔡新忠¹;徐幼平²;楼健²

(¹浙江大学植物保护系, 杭州310029 ;² 浙江大学分析测试中心, 杭州310029)

收稿日期:2002-09-09 修回日期:2003-01-06 出版日期:2003-10-25 发布日期:2003-10-25

Cloning of Tomato myb Gene Fragments and Expression upon Development ofHypersensitive Response

Cai Xinzhong¹;Xu Youping²;Lou Jian²

(¹ Department of Plant Protection , Zhejiang University , Hangzhou 310029 , China ;² Center of Analysis and Measurement , Zhejiang University , Hangzhou 310029 , China)

Received:2002-09-09 Revised:2003-01-06 Online:2003-10-25 Published:2003-10-25

摘要/Abstract

摘要： 以烟草myb1 中MYB 保守区域编码序列的两个相对特异性序列为引物, 以表现过敏性反应
(hypersensitive response , HR) 的番茄苗中提取的RNA 为模板, 通过RT- PCR 获取长度为215 bp 的myb 基因片段LeMYB1 和LeMYB2 (GenBank 核酸序列数据库登录号: AY131230 和AY131231) 。两者只有第63 位碱基不同, 前者序列为A , 而后者为G。Northern 杂交分析表明, LeMYB 相关基因表达时序与番茄苗HR 的产生和发展呈密切正相关, 因而可能在Cf-9 决定的HR 和抗病性产生中起调节作用。

关键词: 番茄, LeMYB, 转录因子, 基因表达, Cf-9, 过敏性反应

Abstract: Two 215 bp myb gene fragments,LeMYB1 and LeMYB2 with GenBank accession No. AY131230 and AY131231 respectively , were cloned through RT - PCR. The primers were designed in accordance with tobacco myb1 at the DNA-binding domain conserved in MYB , and the template was the RNA isolated from tomato seedlings showing hypersensitive responses (HR) . Only nucleotide 63 was different between the two fragments , of which LeMYB1 was A and LeMYB2 was G. Three nucleotides within the primers for cloning of LeMYB1 were different between LeMYB1 and counterpart of THM18,whereas 90.7% nucleotide sequence of LeMYB1 was identical to corresponding parts of tobacco myb1. Northern assay revealed that expression of LeMYB1-related genes were
induced upon occurrence of hypersensitive necrosis in tomato seedlings and enhanced henceforth. Their coincident expression with HR development in tomato indicated that LeMYB1-related genes might be involved in regulation of
HR and disease resistance conferred by tomato fungal resistance gene Cf-9.

Key words: Tomato, LeMYB, Transcription factor, Gene expression, Cf-9, Hypersensitive response

中图分类号:

S 641.2

蔡新忠;徐幼平;楼健. 番茄myb基因片段的克隆及在过敏性反应时的表达[J]. 园艺学报, 2003, 30(5): 589-591.

Cai Xinzhong;Xu Youping;Lou Jian. Cloning of Tomato myb Gene Fragments and Expression upon Development ofHypersensitive Response[J]. ACTA HORTICULTURAE SINICA, 2003, 30(5): 589-591.

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