用AFLP分析小金海棠的起源

园艺学报 ›› 2008, Vol. 35 ›› Issue (2): 281-284.

用AFLP分析小金海棠的起源

石胜友¹ ;成明昊² ;胡玉林¹ ;郭启高² ;梁国鲁^2* ;周志钦^2*

（¹中国热带农业科学院南亚热带作物研究所，广东湛江 524091； ² 西南大学园艺园林学院，重庆 400716）

收稿日期:2007-08-08 修回日期:2008-01-11 出版日期:2008-02-25 发布日期:2008-02-25
通讯作者: 梁国鲁;周志钦

Application of AFLP Analysis in Origin of Malus xiaojinensis Cheng et Jiang

SHI Sheng-you¹，Cheng Ming-hao²，HU Yu-lin¹，GUO Qi-gao²，LIANG Guo-lu^2*，and ZHOU Zhi-qin^2*

(¹ South Subtropical Crop Research Institute, Zhanjiang, Guangdong 524091，China;²College of Horticulture and Landscape, Southwest University , Chongqing 400715，China)

Received:2007-08-08 Revised:2008-01-11 Online:2008-02-25 Published:2008-02-25
Contact: LIANG Guo-lu,ZHOU Zhi-qin

摘要/Abstract

摘要： 对小金海棠、变叶海棠居群内分化出的似小金海棠的植株、变叶海棠×陇东海棠（似小金海棠）、陇东海棠、变叶海棠进行了AFLP分析。结果表明：小金海棠、变叶海棠居群内分化出的似小金海棠的植株和变叶海棠×陇东海棠（似小金海棠）具有高度的遗传一致性，相似性系数为0.977～0.986；小金海棠与陇东海棠和变叶海棠之间有相近的亲缘关系，小金海棠的谱带有98.08%是来自于变叶海棠和陇东海棠的叠加。该研究表明小金海棠是变叶海棠物种分化及陇东海棠种质渗入所形成的多倍体杂种。

关键词: 小金海棠, AFLP, 遗传一致性, 相似性系数, 起源, 杂交

Abstract:

This study analyzed Malus xiaojinensis Cheng et Jiang, apple trees were similar to Malus xiaojinensis Cheng et Jiang differentiated from M. toringoides Hughes, M. toringoides Hughes×M. kansuensis Rehd, M. kansuensis Rehd, M. toringoides Hughes by AFLP to explore the origin of M.xiaojinensis Cheng et Jiang. There were high degree of genetic identity among M.xiaojinensis Cheng et Jiang, apple trees were similar to M. xiaojinensis Cheng et Jiang differentiated from M. toringoides Hughes and M. toringoides Hughes×M. kansuensis Rehd, and their genetic similar coefficient were from 0.977 to 0.986. M. xiaojinensis Cheng et Jiang, meanwhile there were 98.08% bands of M. xiaojinensis Cheng et Jiang were from M. kansuensis Rehd and M. toringoides Hughes. The results showed that M. kansuensis Rehd and M. toringoides Hughes had close relationship. According to the AFLP analysis and previous researchs, we discussed the origin of M. xiaojinensis Cheng et Jiang and proposed that the species may derive from M. toringoides Hughes×M. kansuensis Rehd. Our findings have provided basic research data and important directions for the cultivation and exploitation of the Malus xiaojinensis.

Key words: Malus xiaojinensis Cheng et Jiang, AFLP, Genetic identity, Genetic similar coefficient, origin, hybrid

中图分类号:

S 661

石胜友;成明昊;胡玉林;郭启高;梁国鲁;周志钦 . 用AFLP分析小金海棠的起源[J]. 园艺学报, 2008, 35(2): 281-284.

SHI Sheng-you;Cheng Ming-hao;HU Yu-lin;GUO Qi-gao;LIANG Guo-lu;and ZHOU Zhi-qin . Application of AFLP Analysis in Origin of Malus xiaojinensis Cheng et Jiang [J]. ACTA HORTICULTURAE SINICA, 2008, 35(2): 281-284.

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