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园艺学报 ›› 2003, Vol. 30 ›› Issue (5): 545-548.

• 研究论文 • 上一篇    下一篇

培养基对胡萝卜悬浮系茄红素合成代谢活性的影响

梁燕1;陈大明2*;王鸣1;陈杭2   

  1. (1西北农林科技大学园艺学院, 杨凌712100;2北京市农林科学院蔬菜研究中心, 北京100089)
  • 收稿日期:2003-04-07 修回日期:2003-07-25 出版日期:2003-10-25 发布日期:2003-10-25

Effects of Culture Medium Factors on Lycopene Synthesis in Carrot SuspensionCells

Liang Yan1;Chen Daming2*;Wang Ming1;Chen Hang2   

  1. (1 Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100 , China;2 Beijing Vegetable Research Center , Beijing100081 , China)
  • Received:2003-04-07 Revised:2003-07-25 Online:2003-10-25 Published:2003-10-25

摘要: 以胡萝卜品种‘新透心红’稳定的细胞悬浮系为材料, 研究了基本培养基、蔗糖浓度和PO43-浓度对其茄红素合成代谢活性的影响。结果表明, 在B5 、MG5 、ER 和White 培养基中, MG5 培养基最适宜于茄红素合成代谢, 细胞中茄红素含量显著高于其它培养基。以MG5 为基本培养基, 在7 个蔗糖浓度处理中, 100.0~120.0 g·L-1范围内, 细胞中茄红素含量极显著高于其它处理, 茄红素合成代谢活性最高; 在13个PO43-浓度处理中, 30.0~50.0 mmol·L-1范围内, 细胞中茄红素含量极显著高于其它处理, 茄红素合成效果最佳。

关键词: 胡萝卜, 培养基, 蔗糖浓度, PO43- 浓度, 茄红素合成, 悬浮系

Abstract: Taking a constant cell suspension systemof carrot ( Daucus carota L. ) variety -Xin Touxinhong as material , the effects of basic medium , sucrose and PO43- concentrations on lycopene synthesis activity of carrot suspension cells were studied by measurement and significant analysis on lycopene content of fresh cells per unit weight (in terms of light absorption values at 470 nm) among different treatments. The results showed that the lycopene content in carrot suspension cells under MG5 medium was significantly higher than those under the other media , MG5 was the most favorable basic medium for lycopene synthesis of carrot cells among MG5 , ER , B5 and White ; on the basis of MG5 medium , the lycopene contents under 100.0 - 120.0 g·L-1 of sucrose concentration among 7 treatments and 30.0 - 50.0 mmol·L-1 of PO43-concentration among 13 treatments were very significantly higher than those of the other corresponding treatments , and better for lycopene synthesis of carrot cells

Key words: Daucus carota L., Sucrose concentration, PO43- concentration, Lycopene synthesis, Cell suspension system

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