关键词: 苹果砧木, SH系, SSR标记

Abstract: The phenomenon of homonym and synonym of SH series apple rootstocks happened in the process of introduction breeding, which was also proved in the practice of planting. Therefore, it was necessary to clarify stem and series of homonym and synonym. With 28 SH series apple rootstocks preserved in Pomology Insitute of Shanxi(Shanxi), Institute of Forestry and Pomology of Beijing (Beijing) and Institute of Pomology of Changping, Beijing (Changping, Beijing) as materials,used method of simple sequence repeat (SSR) molecular markers, screened 82 pairs primer from 112 pairs to conduct PCR amplification, analyzed results of polyacrylamide gel electrophoresis and conducted data analysis of bands data matrices by NTSYS software. 〖JP2〗The results showed that among homogeneity in different institutes, SH9 in Shanxi and Beijing has 100% similarity; There were four homonyms in the samples, i.e. the similarity coefficients of SH17, SH18, SH40 coming from different conservative places were as low as 68.9%, 55.1% and 65.6% respectively and among the similarity coefficients of SH38 coming from different conservative places the highest was 61.2%. Three heteronyms were also identified: SH18 (Shanxi) and SHb(100%), SH17 (Changping, Beijing) and SHd(99.5%), SHa and SHe(99.5%), SH18(28) and SH40 (Shanxi) (99%), all of them had a similarity coefficient larger than 99%. In addition, high similarities were found between SH18 and SH38 from Changping (98.4%) and among SH12,SH17(Shanxi)and SH29 ( 97.2%-98.9%). SH1, SH3, SH6, SH8-11, SH9-hf, SH19, SH28, SH29, SHc, SHd and SHf etc. in different conservative places were heterogeneous.

Key words: apple rootstock, SH series, SSR marker