一个中国水仙MADS-box基因的克隆与分析

园艺学报 ›› 2008, Vol. 35 ›› Issue (2): 295-300.

一个中国水仙MADS-box基因的克隆与分析

高志民1，陈段芬1，李雪平1，蔡春菊1，彭镇华2*

（1 国际竹藤网络中心，国家林业局竹藤科学与技术重点开放实验室，北京 100102；2 中国林业科学研究院林业研究所，北京 100091）

收稿日期:2007-07-16 修回日期:2007-09-20 出版日期:2008-02-25 发布日期:2008-02-25

Cloning and Sequence Analysis of a Flowering-related MADS-box Gene in Narcissus tazetta var. chinensis

GAO Zhi-min1, CHEN Duan-fen1, LI Xue-ping1, CAI Chunju1 and PENG Zhen-hua*2

(1 International Center for Bamboo and Rattan, SFA key Laboratory of Bamboo and Rattan Science and Technology, Beijing 100102, China；2 Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China)

Received:2007-07-16 Revised:2007-09-20 Online:2008-02-25 Published:2008-02-25

摘要/Abstract

摘要： 采用RT-PCR技术从中国水仙（Narcissus tazetta var.chinensis）幼嫩花蕾中分离到一个新的水仙花发育相关MADS-box基因，命名为NTMADS1（GenBank登记号：EF421828）。该基因长879 bp，编码区共编码230个氨基酸，具有典型的植物MADS-box基因结构，由MADS区、I区、K区和C末端组成。序列分析结果表明，NTMADS1编码的蛋白与其它植物的MADS-box蛋白有着较高的一致性，其中与扫状天门冬（Asparagus virgatus）的AVAG1的一致性高达89％。系统进化树分析表明NTMADS1属于AG亚族。

关键词: 中国水仙, MADS-box 基因, 系统进化树分析

Abstract:

MADS-box genes play important role in various plant development processes，especially in the development of floral organs．The flowering-related MADS-box gene, named as NTMADS1 (GenBank accession number：EF421828), was cloned from the first strand of N. tazetta var. chinensis cDNA through RT-PCR using primers designed according to the MADS-box gene family conserved region. The fragment was 879 bp long and contained an open reading frame of 693 bp coding a polypeptide of 230 amino acids which had a typical MADS-box domain. Homology analysis showed that the deduced NTMADS1 protein was highly homologous to other MADS-box proteins from different species, especially with Asparagus virgatus to 89%. Phylogenetic tree analysis also indicated that NTMADS1 belongs to AG subfamily.

Key words: Narcissus tazetta var. chinensis, MADS-box gene, Phylogenetic tree analysis

中图分类号:

S 682.2

高志民;陈段芬;李雪平;蔡春菊;彭镇华. 一个中国水仙MADS-box基因的克隆与分析 [J]. 园艺学报, 2008, 35(2): 295-300.

GAO Zhi-min;CHEN Duan-fen;LI Xue-ping;CAI Chunju and PENG Zhen-hua. Cloning and Sequence Analysis of a Flowering-related MADS-box Gene in Narcissus tazetta var. chinensis[J]. ACTA HORTICULTURAE SINICA, 2008, 35(2): 295-300.

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