关键词: 黄瓜, 高温, 花药, 钙, ABA, 蛋白质双向电泳

Abstract: Under 45℃ treatment, Ca²⁺ precipitates in pollen and anther cells increase more quickly in cucumber (Cucumis sativus L. ) heat-sensitive line Xintaimici than in heat-tolerant line JY24. While the temperature recover to 28℃, the Ca²⁺ precipitates quantity drop to the normal levelmore tardily in Xitaimici
anther cells. EndogenousABA contents in two line anthers also ascend significantly after 1 h 45℃ treatment,especially in Xintaimici. When the temperature return to 28℃, ABA in Xitaimici anthers step down to the
control level, while JY24 anthers can maintain a higher ABA content within 2 h. No any additional protein points can be seen in the IEF-SDS-PAGE maps of cucumber anthers under 45℃ stress comparing with 28℃, although the expression of some proteins, such as (MW 70 kD, P I 5.7) and (MW 45.5 kD, PI 5.6) wereup regulated remarkably after heat treatment ( even higher in JY24 than in Xintaimici). The results indicated both Ca²⁺ and ABA signalswere involved in the response of cucumber anthers to heat-stress. The synthesis of
HSPs in cucumber uninucleate-binucleate-pollen-phase antherswere at least in part inhibited under 45℃.

Key words: Cucumber, High temperature, Anther, Ca^{2 +}, ABA, 2D PAGE