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园艺学报 ›› 2007, Vol. 34 ›› Issue (3): 597-604.

• 果树 • 上一篇    下一篇

东北草莓×凤梨草莓种间杂种一代的细胞遗传学观察与RAPD分析

马鸿翔1*; 陈佩度2; 余桂红1; 任丽娟1   

  1. (1 江苏省农业科学院农业生物遗传生理研究所, 南京210014; 2 南京农业大学作物遗传与种质创新国家重点实验室,南京210095)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-06-25 发布日期:2007-06-25

Cytogenetics and RAPD Analysis of Interspecific Hybrids from the Cross ofFragaria mandschurica Staudt and F. ananassa Duch.

MA Hong-xiang1*; CHEN Pei-du2;YU Gui-hong1 ;REN L i-juan1   

  1. (1 Institute of Agro-biological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

摘要: 以利用我国原产草莓二倍体野生种东北草莓与八倍体栽培种凤梨草莓栽培品种‘圣星’杂
交, 通过胚拯救获得的种间杂种为试材, 经体细胞鉴定种间杂交后代为五倍体, 育性差。叶柄茸毛和花梗
茸毛与野生种母本东北草莓相近; 花序着生高度部分与母本一致, 部分与父本一致, 各占一半; 植株高度
多数介于双亲之间, 趋向于栽培品种。杂交F1代的花粉母细胞减数分裂观察表明, 染色体构型为6.1Ⅰ +
10.6Ⅱ + 2.1Ⅲ + 0.35Ⅳ, 后期出现少量畸形多分孢子。RAPD分析表明, 杂种后代具有亲本扩增片段, 后
代个体间存在遗传异质性。

关键词: 草莓, 东北草莓, 凤梨草莓, 种间杂交, 细胞遗传学, RAPD

Abstract: The interspecific hybrid was obtained from the cross of Fragaria mandschurica Staudt, a
dip loid species derived from Northeast China, and F. ananassa Duch. (2n = 8x = 56) ‘Shengxing’by using
immature embryo culture technique. The hybridswere pentap loidswith poor fertility. The morphological char
acteristics of the hybridswere observed and showed that theywere intermediate between their parents in pedun
cle height and p lant height, and similar to Fragaria m andschurica in hair on peduncle and petiole. The
hybrids showed expected 35 chromosomes in somatic cells and had a meiotic chromosome association of 6.1Ⅰ
+ 10.6 Ⅱ + 2.1 Ⅲ + 0.35 Ⅳ with a few abnormal microspores occurring in tetrad phase. RAPD analysis
showed that the hybrids possessed the specific bands of two parents, and the genetic diversities were found
among the interspecific hybrid lines.

Key words: Strawberry, Fragaria mandschurica Staudt, Fragaria ananassa Duch., Interspecifichybridization, Cytogenetics, RAPD