新高系梨雄性不育的鉴定

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 289-294.

新高系梨雄性不育的鉴定

袁德义¹;谭晓风^1*;张　琳¹;何小勇¹;赵思东¹;乌云塔娜¹;段经华¹;李秀根2

(¹中南林业科技大学经济林育种与栽培国家林业局重点实验室, 长沙410004; ²中国农业科学院郑州果树研究所, 郑州450009)

收稿日期:2006-11-03 修回日期:2007-03-12 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 谭晓风

Identification of Male Sterility of Pear Cultivars in Niitaka Line

YUAN De-yi¹, TAN Xiao-feng^1*, ZHANG Lin¹, HE Xiao-yong¹,ZHAO Si-dong¹, WUYUN Ta-na¹,DUAN Jing-hua¹, and Li Xiu-gen²

(¹The Key Laboratory of Non-wood Forestry Product of the Ministry of Forestry, Central South Forestry University, Changsha 410004, China; ²Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China)

Received:2006-11-03 Revised:2007-03-12 Online:2007-04-25 Published:2007-04-25
Contact: TAN Xiao-feng

摘要/Abstract

摘要： 在砂梨( Pyrus pyrifolia Nakai) 中首次发现新高系梨绝大多数品种为雄性不育品种(品系) ,
运用5种方法对其进行了鉴定: 形态学观测花药、花粉情况; 花粉离体培养法; 整体染色透明法对花药显微观测; 石蜡切片解剖法对大蕾期花药、花粉囊、花粉显微观测; 田间授粉试验。结果表明: 新高系梨9个品种中除‘早生黄金’梨为雄性可育品种外, 其它8个品种即‘新高’、‘黄金’、‘水晶’、‘荣山’、‘天皇’、‘金秋’、‘晚大新高’、‘农家新高’均无正常花粉或正常花粉极少, 为花粉败育, 属孢子发生性雄性不育类型, 且其雄性不育程度存在一定差异。

关键词: 砂梨, 新高系, 雄性不育, 品种, 鉴定

Abstract: A large number of Niitaka line cultivars in sand pear (Pyrus pyrifolia Nakai) exhibited male sterility were firstly found. Nine cultivars in Niitaka line were examined by morphologic observation of pollen and anther, pollen culture in vitro, whole stain2chearing technique, paraffin sectioning, and pollination tests. The results indicated that Josengwhangkeum was a male fertile cultivar. All other eight cultivars, Niitaka,WhangKeumBae, Sujeong, Yeongsanbae, Yewang, J inqiu, Banndainiitaka, and Nokaniitaka, were male sterile cultivars. These p lants had abnormal or extremely few normal pollens that resulted from pollen abortion.Male sterile cultivars in Niitaka line were sporogenesismale sterility. The degree ofmale sterilitywas different among the cultivars.

Key words: Pyrus pyrifolia, Niitaka line, Male sterility, Cultivar, Identification

中图分类号:

S 661.2

袁德义;谭晓风;张　琳;何小勇;赵思东;乌云塔娜;段经华;李秀根. 新高系梨雄性不育的鉴定[J]. 园艺学报, 2007, 34(2): 289-294.

YUAN De-yi;TAN Xiao-feng;ZHANG Lin;HE Xiao-yong;ZHAO Si-dong;WUYUN Ta-na;DUAN Jing-hua;and Li Xiu-gen. Identification of Male Sterility of Pear Cultivars in Niitaka Line[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 289-294.

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