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园艺学报 ›› 2007, Vol. 34 ›› Issue (3): 644-648.

• 蔬菜 • 上一篇    下一篇

不同结构的外源ACO 基因导入番茄对乙烯生成速率的影响

陈银华1,3 ; 李汉霞2 ; 叶志彪1,2*
  

  1. (1 华中农业大学作物遗传改良国家重点实验室, 武汉430070; 2华中农业大学国家蔬菜中心华中分中心, 武汉430070; 3 教育部热带海洋与陆生生物资源研究及利用重点实验室, 海口570228)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-06-25 发布日期:2007-06-25

Effects of Introduction of Different T-DNA Structures of ACO Gene to Tomato Genome on Ethylene Production Rate

CHEN Yin-hua1,3 ; L IHan-xia2 ;YE Zhi-biao1,2*   

  1. 1National Key Laboratory of Crop Genetic Improvem ent, Huazhong Agricultural University, Wuhan 430070, China; 2NationalCenter for Vegetable Improvement (Central China) , Huazhong Agricultural University, W uhan 430070, China; 3 Key Laborotoryfor Tropical Biological Resources, M inistry of Education, Haikou 570228, China〕
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

摘要: 为研究不同结构的外源基因对内源基因的影响, 通过农杆菌介导的遗传转化法将3种不同结
构的外源ACO基因导入番茄基因组中, 对3种不同载体转基因植株的乙烯生成情况进行了检测。结果表
明, 3种载体中, RNAi对内源ACO基因表达抑制作用最明显。

关键词: 番茄, ACO基因, RNAi, 乙烯释放量

Abstract: To analyze the effect of different structures of foreign genes on the endogenous genes, three
different T2DNA structures of ACO gene were introduced into tomato genome through the method of Agrobac
turium 2mediated transformation. The ethylene evolution of the transgenic tomato p lants was measured by gas
chromatography. The results showed that in the three different transgenic p lants the maximum inhibition of en
dogenous ACO gene exp ression was observed using RNAi vectors.

Key words: Tomato, ACO gene, RNAi, Ethylene evolution