部分桂花栽培品种的AFLP分析

园艺学报 ›› 2008, Vol. 35 ›› Issue (1): 137-142.

部分桂花栽培品种的AFLP分析

韩远记¹;董美芳¹;袁王俊²;李瑾¹;韩洁¹;尚富德^1*

（¹河南大学生命科学学院，农业生物技术研究所，河南开封 475001；²河南大学药学院，河南开封 475001）

收稿日期:2007-08-01 修回日期:2007-10-08 出版日期:2008-01-25 发布日期:2008-01-25
通讯作者: 尚富德

Study on the Genetic Diversity of Some Osmanthus fragrans Cultivars Based on AFLP Markers

HAN Yuan-ji¹, DONG Mei-fang¹, YUAN Wang-jun², LI Jin¹, HAN Jie¹, and SHANG Fu-de^1*

(¹College of Life Sciences and Institute of Agricultural Biotechnology, Henan University, Kaifeng, Henan 475001, China; ²College of Pharmaceutical Sciences, Henan University, Kaifeng, Henan 475001, China )

Received:2007-08-01 Revised:2007-10-08 Online:2008-01-25 Published:2008-01-25

摘要/Abstract

摘要： 从22对AFLP引物中筛选出6对用来检测22个桂花品种和木犀属3个种的多态性位点，共检测到171个位点，其中多态性位点104个，占60.8%。利用DPSv 3.11软件计算25个样品之间的Nei遗传距离，并按照非加权算术平均数聚类方法（UPGMA），构建树状分支图。AFLP分析结果表明：桂花花色较深的品种之间和花色较浅的品种之间分别存在着较近的亲缘关系，而花色深浅不同的两类品种之间亲缘关系较远。从聚类图上看，22个桂花品种中最后聚在一起的分别是3个银桂品种（遗传距离0.19处）和3个四季桂品种（遗传距离0.21处），说明四季桂类和银桂类中的部分品种与金桂品种群和丹桂品种群有较远的亲缘关系。从分类上看，AFLP分析结果与传统的以形态特征为基础的分类结果并不完全一致。

关键词: 桂花, 木犀属, 种, AFLP, 亲缘关系

Abstract: 22 primer combinations were used for polymorphic selection, 6 of which could generate reproducible polymorphic fragments and were used to detect the polymorphic sites of 22 Osmanthus fragrans cultivars and 3 species of Osmanthus. A total of 171 bands amplified from 6 primer combinations were selected for AFLP analysis, 104 bands appeared to be polymorphic(60.8%).The data were used to calculate the Nei's genetic distance of the 25 materials and to construct a dendrogram based on UPGMA cluster analysis.The result showed that:It had closely relationship between Osmanthus fragrans cultivars with brunet flower as well as Osmanthus fragrans cultivars with blonde flower,but the relationship between the two groups were remote.From the dendrogram,we knew that some Osmanthus fragrans cultivars of Latifolius Group and Fragrans Group had remotely relationship comparing with Thunbergii Group and Aurantiacus Group.AFLP analysis appeared the taxonomic result was not completely consistent with that of traditional analysis.

Key words: Osmanthus fragrans Lour., Osmanthus, species, AFLP, genetic relationship

中图分类号:

S 685.13

韩远记;董美芳;袁王俊;李瑾;韩洁;尚富德. 部分桂花栽培品种的AFLP分析[J]. 园艺学报, 2008, 35(1): 137-142.

HAN Yuan-ji;DONG Mei-fang;YUAN Wang-jun;LI Jin;HAN Jie;and SHANG Fu-de. Study on the Genetic Diversity of Some Osmanthus fragrans Cultivars Based on AFLP Markers [J]. ACTA HORTICULTURAE SINICA, 2008, 35(1): 137-142.

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