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园艺学报 ›› 2003, Vol. 30 ›› Issue (1): 34-38.

• 研究论文 • 上一篇    下一篇

番茄ss基因的连锁分析及其种子发芽特性

李君明;周永健;徐和金;杨宝军   

  1. (中国农业科学院蔬菜花卉研究所, 北京100081)
  • 收稿日期:2002-01-11 修回日期:2002-09-09 出版日期:2003-02-25 发布日期:2003-02-25

The Linkage Analysis of ss Gene and Its Effect on Seed Germination in Tomato ( Lycopersicon esculentum Mill . )

Li Junming;Zhou Yongjian;Xu Hejin;Yang Baojun   

  1. ( Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences , Beijing 100081 , China)
  • Received:2002-01-11 Revised:2002-09-09 Online:2003-02-25 Published:2003-02-25

摘要: 证明控制番茄种皮表现为海绵状的基因ss 符合孟德尔遗传规律, 并与幼苗绿茎基因ah 连锁,
位于第9 条染色体上, 使种皮表现为灰白色。来自95148 ×红100 的F2 代分离群体的种子, 含有ah 基因的无花青素单株个体较有花青素个体普遍表现为前期(39 或51 h) 发芽率较高, 而第10 天的平均总发芽率却呈现下降趋势; 不同单株个体间差异较大, 总发芽率变化幅度为54 %~100 % , 表明虽然ah 基因可促进番茄种子的前期发芽, 但还受种子基因型, 尤其是胚或胚乳基因型的影响。番茄种子种皮褐色色素的积累和ah、aw 、aa 等基因无关。种皮颜色和茸毛等性状可以作为明显的形态标记, 直接用于后代个体的选择。

关键词: 番茄, ss 基因, 连锁分析, 发芽

Abstract: The genetic mechanism of ss gene controlling expression of spongy seedcoat was proved to fit Mendelian fashion in this paper. It was linked with ah , which showed obvious anthocyaninless ( Hoffman) during seedling stage and located on chromosome 9. Its seedcoat also showed the typical gray white color. The anthocyaninless individuals derived from progeny of the F2 crossing between 95148 as female parent and Hong 100 as male parent showed higher germinating rate respectively in 39 and 51 hours compared to these individuals with anthocyanin.But the great difference existed among these anthocyaninless individuals which also gave the tendency with low total
germinating rate in 10 days. The germinating rate in 10 days in F2 population varied from 54 % to 100 %. The results showed that ah gene could improve the prophase seed germinating but it still depended on the seed genotype ,
especially that of the embryo or endosperm. Further , the accumulation of brown pigment in seedcoat was not related with ahaw and aa genes. The described characterization of seedcoat in this paper could be directly used to select the progenies as a visual morphological marker.

Key words: Tomato, ss gene, Linkage analysis, Germination

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