离体培养诱导多头型苏铁

园艺学报 ›› 2007, Vol. 34 ›› Issue (6): 1575-1578.

离体培养诱导多头型苏铁

林庆良^1*;施榕斌²;林永灿²;林晓白²;龚金才²;邹娜³

(¹福建农林大学园艺学院,福州350002; ²福州市建新花卉集团公司, 福州350002;³福建农林大学林学院,福州350002)

收稿日期:2007-07-20 修回日期:2007-10-08 出版日期:2007-12-25 发布日期:2007-12-25

Plantlets with Multi-shoots of Cycas revoluta Thunb in Vitro

LIN Qing- liang^1*,SHI Rong-bin²,LIN Yong-can², LIN Xiao-bai²,GONG Jin-cai²,and ZOU Na³

(¹College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002,China;²Jianxin Flower Group Company of Fuzhou ,Fuzhou 350002,China;³College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002,China)

Received:2007-07-20 Revised:2007-10-08 Online:2007-12-25 Published:2007-12-25

摘要/Abstract

摘要： 进行了苏铁种子、子叶、鳞片、顶芽的试管育苗和多头苏铁诱导的研究。结果表明：试管播种的种子比常规播种提早发芽6个月，发芽率高；在ER＋BA 2 mg/L＋IBA 0.4 mg/L＋苏铁外种皮汁2 mL/L培养基中，多头诱导率达76.7%。离体培养的子叶和鳞片的芽诱导率分别达到53.3%和33.3%；试管苗在4～7月份移栽为好，成活率达90%以上。

关键词: 苏铁, 多头型, 离体培养

Abstract: The plantlet regeneration, with multi-shoots especially, derived from seed, cotyledon, squama and tip-bud of Cycas revolute Thunb was studied in vitro. The results showed that the germination of seed cultured in vitro could be six months earlier than that of sowing in normal condition. When inoculated on ER＋BA 2 mg/L＋IBA 0.4 mg/L＋juice of Cycas revolute Thunb episperm 2 ml/L,the rate of multi-shoots was 76.7%. The differentiation rate of bud derived from cotyledon and squama was 53.3% and 33.3% , respectively. It will be better when plantlets were transplanted from April to July; and the survival rate was more than 90%.

Key words: Cycas revoluta, Multi-shoot, In vitro culture

中图分类号:

S 68

林庆良;施榕斌;林永灿;林晓白;龚金才;邹娜. 离体培养诱导多头型苏铁[J]. 园艺学报, 2007, 34(6): 1575-1578.

LIN Qing- liang;SHI Rong-bin;LIN Yong-can;LIN Xiao-bai;GONG Jin-cai;and ZOU Na. Plantlets with Multi-shoots of Cycas revoluta Thunb in Vitro[J]. ACTA HORTICULTURAE SINICA, 2007, 34(6): 1575-1578.

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