关键词: 草莓, 草莓镶脉病毒, 检测, PCR

Abstract: Total DNA , isolated from 30 strawberry cultivars , was used as template to amplify strawberry vein banding virus (SVBV) CP gene fragment by polymerase chain reaction (PCR) . Meanwhile , the tested plants were detected by indicator leaf grafting technique.The plants with typical band in PCR showed vein banding symptoms in indicator UC5 ( Fragaria vesca) , EMC ( F. vesca) , and those without typical band didn’t showed symptoms. Results were identical in two detection methods. The amplified fragment by PCR was recycled , cloned in pMD18-T vector , and then sequenced. It is suggested that the expected SVBV CP gene fragment was obtained and is 90. 94 % identical to that of American isolate ATCC 45058.

Key words: Strawberry, Strawberry vein banding virus (SVBV), Detection, PCR