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园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 497-500.

• 研究简报 • 上一篇    下一篇

单倍体甜瓜离体繁殖的方法

张永兵1,2;伊鸿平2;贾媛媛1;冯炯鑫2;吴明珠2;陈劲枫1*   

  1. (1作物遗传与种质创新国家重点实验室,南京农业大学园艺学院,南京 210095;2新疆农业科学院哈密瓜研究中心,乌鲁木齐 830000)
  • 收稿日期:2006-11-16 修回日期:2007-03-12 出版日期:2007-04-25 发布日期:2007-04-25
  • 通讯作者: 陈劲枫

Propagation of Haploid Melon (Cucumis melo L.) in Vitro

ZHANG Yong-bing1,2, YI Hong-ping2, JIA Yuan-yuan1, FENG Jiong-xin2, WU Ming-zhu2, and CHEN Jin-feng1*   

  1. (1State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China; 2Center of Hami Melon, Xinjiang Academy of Agricultural Science, Wurumuqi, 830000, China)
  • Received:2006-11-16 Revised:2007-03-12 Online:2007-04-25 Published:2007-04-25
  • Contact: CHEN Jin-feng

摘要:

以单倍体甜瓜无菌苗的顶芽和腋芽为外植体进行离体繁殖研究。结果表明:单倍体甜瓜最佳增殖培养基为MS + 6-BA 0.5mg·L-1,最适生根培养基为1/2MS + IBA 0.5mg·L-1,增殖系数为3.57。染色体计数表明没有倍性变化发生。

关键词: 甜瓜, 单倍体, 离体繁殖

Abstract: The propagation in vitro of haploid melon was investigated using shoot-tips and lateral buds from haploid melon plantlets. The results indicated that the optimal media for shoot proliferation was MS + 6-BA 0.5mg·L-1 and the optimal media for rooting was 1/2MS + IBA 0.5mg·L-1. The multiplication coefficient was 3.57. The chromosome number was checked and no ploidy variation occurred among these plants.

Key words: Melon, Haploid, Propagation in vitro

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