怀地黄玻璃化和包埋玻璃化法超低温保存

园艺学报 ›› 2008, Vol. 35 ›› Issue (4): 607-610.

怀地黄玻璃化和包埋玻璃化法超低温保存

李明军*;周娜;刘杰;张晓丽;李萍;张楠

（河南师范大学生命科学学院，河南新乡 453007）

收稿日期:2007-12-29 修回日期:2008-02-27 出版日期:2008-04-25 发布日期:2008-04-25
通讯作者: 李明军

Cryopreservation of Rehmannia glutinosa (Gaertn.) Libosch by Vitrification and Encapsulation-vitrification

LI Ming-jun*, ZHOU Na, LIU Jie, ZHANG Xiao-li, LI Ping, and ZHANG Nan

（College of Life Sciences, Henan Normal University, Xinxiang，Henan 453007, China）

Received:2007-12-29 Revised:2008-02-27 Online:2008-04-25 Published:2008-04-25
Contact: LI Ming-jun

摘要/Abstract

摘要：

比较了玻璃化与包埋玻璃化超低温保存怀地黄（Rehmannia glutinosa(Gaertn.) Libosch）‘85-5’带芽茎段的效果。结果表明：4 ℃低温锻炼5 d，在添加二甲基亚砜（DMSO）和乙酰胺的预培养基中预培养3 d，60 % PVS₂室温装载25 min，PVS₂ 0 ℃脱水30 min时，玻璃化和包埋玻璃化两种保存方法均达到本试验条件下最佳结果；两种方法相比，玻璃化法的存活率和再生率均较包埋玻璃化法高，前者的存活率（88.92 %）和再生率（64.29 %）分别是后者的1.38倍和2.41倍。可见，采用超低温保存怀地黄种质资源是可行的，且玻璃化法优于包埋玻璃化法。

关键词: 怀地黄, 带芽茎段, 玻璃化, 包埋玻璃化, 超低温保存

Abstract:

The cryopreservation methods of Rehmannia glutinosa (Gaertn.) Libosch by vitrification and encapsulation-vitrification were studied. The results showed that: it was optimal cryopreservation condition for the two methods in the experimentation when the materials were trained at 4 ℃ low temperature for 5 d, then pre-cultured on the medium with DMSO and Acetamide for 3 d, loaded with 60 % PVS₂ for 25 min at room temperature and dehydrated with PVS2 for 30 min at 0 ℃. But compared with the materials cyropreserved by the two methods, the survival rate and regeneration rate by vitrification were both higher than those by encapsulation-vitrification. The survival rate (88.92 %) and the regeneration rate (64.29％) of the former were 1.38 times and 2.41 times higher than the latter, suggesting cryopreservation of Rehmannia glutinosa (Gaertn.) Libosch by vitrification is applicable to the germplasm conservation.

Key words: Rehmannia glutinosa (Gaertn.) Libosch, stem with bud, vitrification, encapsulation- vitrification, cryopreservation

中图分类号:

S 567.23⁺4

李明军;周娜;刘杰;张晓丽;李萍;张楠. 怀地黄玻璃化和包埋玻璃化法超低温保存[J]. 园艺学报, 2008, 35(4): 607-610.

LI Ming-jun;ZHOU Na;LIU Jie;ZHANG Xiao-li;LI Ping;and ZHANG Nan. Cryopreservation of Rehmannia glutinosa (Gaertn.) Libosch by Vitrification and Encapsulation-vitrification[J]. ACTA HORTICULTURAE SINICA, 2008, 35(4): 607-610.

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