中国野生葡萄果实抗白腐病基因的分子标记

园艺学报 ›› 2003, Vol. 30 ›› Issue (1): 6-10.

中国野生葡萄果实抗白腐病基因的分子标记

徐炎¹;王跃进^1,*;周鹏²;张剑侠¹

(¹西北农林科技大学园艺学院, 杨凌712100;² 热带农业科学院热带作物生物技术国家重点实验室, 海口571101)

收稿日期:2002-03-26 修回日期:2002-07-22 出版日期:2003-02-25 发布日期:2003-02-25
通讯作者: 王跃进

Identification of Molecular Genetic Markers Tightly Linked to White Rot Resistant Genes in Chinese Wild Grape

Xu Yan¹;Wang Yuejin¹;Zhou Peng²;Zhang Jianxia¹

(¹College of Horticulture , Northwest Sci-Tech university of Agriculture and Forestry , Yangling 712100 , China ;² National Key biotechnology laboratory for tropical cropes , Chinese Academy of Tropical Agricultural Sciences , Haikou 571101 , China)

Received:2002-03-26 Revised:2002-07-22 Online:2003-02-25 Published:2003-02-25

摘要/Abstract

摘要： 以感病×抗病的葡萄种间杂交组合白玉霓×塘尾的F₁代17 个单株及塘尾自交一代16 个单株
为试材, 采用BSA 法和RAPD 技术, 通过对155 个随机引物的筛选, 获得了一个与中国野生葡萄抗白腐病基因连锁的RAPD 标记OPP09-760 , 并在中国野生葡萄8 个种的32 个株系及欧洲葡萄16 个品种中得到验证。进一步将该DNA 片段克隆并测序, 根据两端序列, 设计了一对长26 bp 的特异引物分别扩增供试材料, 获得了与该RAPD 标记相同大小的DNA 片段, 成功地将RAPD 标记转化为SCAR 标记, 可以用作抗白腐病基因的分子辅助选择的依据。

关键词: 葡萄, 中国野生葡萄, 白腐病, RAPD, SCAR, 抗病基因

Abstract: Bulk segregant analysis (BSA) , randomly amplified polymorphic DNA (RAPD) and sequnence characterized amplified region (SCAR) methords were used to tag the white rot-resistant genes of grape with 17 individuals of F₁progeny between Tangwei ( Vitis davidii ) , white rot-resistant and Ugni Blanc (V.vinifera) ,White rot-sensitive and 16 individuals self-pollinated of Tangwei . Among 155 random primers gave distinct band Patterns , One RAPD marker OPP09-760 was tightly linked to a major gene resistant to Coniothyrium diplodiella.Marker OPP09-760 was cloned and sequenced. According to the Sequence, two specific primers were designed to amplify all plant materials. RAPD marker was converted into SCAR marker. One distinct single band only in resistant plants was amplified and its size was the same as that of the RAPD marker. The SCAR marker’s popularity was confirmed. And it could be used for the identification of hybrid resistant to Coniothyrium diplodiella.

Key words: Grape, Chinese wild grape, White rot, RAPD, SCAR, Resistant genes

中图分类号:

徐炎;王跃进;周鹏;张剑侠. 中国野生葡萄果实抗白腐病基因的分子标记[J]. 园艺学报, 2003, 30(1): 6-10.

Xu Yan;Wang Yuejin;Zhou Peng;Zhang Jianxia. Identification of Molecular Genetic Markers Tightly Linked to White Rot Resistant Genes in Chinese Wild Grape[J]. ACTA HORTICULTURAE SINICA, 2003, 30(1): 6-10.

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