杧果主要品种遗传多态性的AFLP标记研究

园艺学报 ›› 2006, Vol. 33 ›› Issue (4): 725-730.

杧果主要品种遗传多态性的AFLP标记研究

雷新涛¹;王家保²;徐雪荣¹;林顺权^3*

(¹中国热带农业科学院南亚热带作物研究所, 广东湛江524091; ² 中国热带农业科学院热带生物技术研究所, 海南海口571101; ³华南农业大学园艺生物技术研究所, 广东广州510640)

收稿日期:2005-08-24 修回日期:2006-01-18 出版日期:2006-08-25 发布日期:2006-08-25

Studies on Genetic Polymorphism of Main Mango Cultivars via AFLP Markers

Lei Xintao¹;Wang Jiabao²;Xu Xuerong¹;Lin Shunquan^3*

(¹Southern Subtropical Crops Research Institute, Chinese Academy of Tropical Agriculture Sciences, Zhanjiang, Guangdong 524091,China; ² Institute of Tropical Bioscience, Chinese Academy of Tropical Agriculture Sciences, Haikou, Hainan 571101, China;³ Institute of B iotechnology in Horticultural Plants, South China Agriculture University, Guangzhou, Guangdong 510640, China)

Received:2005-08-24 Revised:2006-01-18 Online:2006-08-25 Published:2006-08-25

摘要/Abstract

摘要： 采用扩增片段长度多态性AFLP标记对国内外31个杧果主要品种进行分类与亲缘关系研究。结果表明: 筛选出的14对引物组合在31份杧果种质中共扩增出了1 761条带, 其中多态性带的比例为97%。平均每对引物产生125.8条带和121.6条多态性带, 总的多态性带率为97%; 基于AFLP标记, 以0.48的相似系数为阈值, 所有供试　果材料被分为7大组群, 第一组群内以0.52为阈值, 又可分为6组。与形态标记相比, 基于AFLP标记的杧果分类体系更能反映杧果品种间的亲缘关系。

关键词: 杧果, 遗传多态性, AFLP

Abstract: In this article, the classification and the relationship s of 31 main domestic and overseas mango (Mangifera indica Linn. ) cultivars were investigated via amplified fragments length polymorphism (AFLP) markers. The results showed that the 142 pair primer combination screened totally amp lified 1 761 bands from the sampled mango cultivars, and 97% of all bands were polymorphic. For each pair of primers, there were 125.8 bands and 121.6 polymorphic bands and the polymorphic bands rate was 97%. Based on the AFLP markers, all the materials were divided into 7 group swith the threshold of 0148 of similarity coefficient. At the same time, the first group was divided into 6 subgroups with the threshold of 0.52 of similarity coefficient. Compared with morphologic markers, the mango classification system based on AFLP markers could reflect relationship among mango cultivars more efficiently.

Key words: Mango, Genetic polymorphism, AFLP

雷新涛;王家保;徐雪荣;林顺权. 杧果主要品种遗传多态性的AFLP标记研究[J]. 园艺学报, 2006, 33(4): 725-730.

Lei Xintao;Wang Jiabao;Xu Xuerong;Lin Shunquan. Studies on Genetic Polymorphism of Main Mango Cultivars via AFLP Markers[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 725-730.

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