拟南芥花期基因FT 转化切花菊‘神马’

园艺学报 ›› 2010, Vol. 37 ›› Issue (3): 441-448.

拟南芥花期基因FT 转化切花菊‘神马’

姜丹;梁建丽;陈晓丽;洪波;贾文锁;赵梁军

中国农业大学农学与生物技术学院观赏园艺与园林系，北京100193

收稿日期:2009-03-16 修回日期:2010-02-13 出版日期:2010-03-25 发布日期:2010-03-25
通讯作者: 赵梁军

Transformation of Arabidopsis Flowering Gene FT to from Cut Chrysanthemum‘Jinba’by Agrobacterium Mediate

Jiang Dan;Liang Jian-li;Chen Xiao-li;Hong Bo;Jia Wen-suo;Zhao Liang-jun

(Department of Ornamental Horticulture and Landscape Architecture，China Agricultural University，Beijing 100193)

Received:2009-03-16 Revised:2010-02-13 Online:2010-03-25 Published:2010-03-25
Contact: Zhao Liang-jun

摘要/Abstract

摘要： 采用RT-PCR 方法从拟南芥叶片中克隆FT 基因，经过测序分析、酶切之后，连接到植物表达载体Super1300⁺，构建植物重组载体FT- Super1300⁺，运用农杆菌介导法将FT 基因导入切花菊‘神马’中，鉴定其在转化植株体内的整合和表达。扩增得到的基因片段经测序分析与GenBbank 上的FT 基因同源性为100%；构建的植物表达载体经过酶切分析证实外源基因已经正确插入；转化后得到了29 株抗性植株，PCR 和PCR-Southern 杂交结果显示，8 株抗性植株为阳性，说明外源基因整合到转化植株的基因组中。RT-PCR 鉴定结果表明外源基因在转化植株叶片中表达。其中转FT 基因的一个株系在组培条件下分化出花芽，表明转基因植株花芽分化不受光周期影响，可以提前花期。

关键词: 切花菊, FT 花期基因, 转基因, 花期

Abstract: FT gene was cloned by RT-PCR from wild type Arabidopsis. After sequencing, the FT gene with proper digestion sites was digested and inserted into plant expression vector Super1300⁺, and was introduced into Chrysanthemum genome by Agrobacterium-mediated transformation. The integration of
FT was determined using PCR and PCR-Southern, and gene expression was tested by RT-PCR. After transformation, we obtained 29 resistant plants. PCR and PCR-Southern blot analysis showed that eight of them were positive. RT-PCR detected the expression of the exogenous FT gene in the leaves of transgenic plants. One line of eight transplants appeared flowering bud in vitro. It indicated that transplants flowered early without influence of light.

Key words: Cut chrysanthemum, FT gene, transgenic, flowering

中图分类号:

S 682.1⁺3

姜丹;梁建丽;陈晓丽;洪波;贾文锁;赵梁军. 拟南芥花期基因FT 转化切花菊‘神马’[J]. 园艺学报, 2010, 37(3): 441-448.

Jiang Dan;Liang Jian-li;Chen Xiao-li;Hong Bo;Jia Wen-suo;Zhao Liang-jun. Transformation of Arabidopsis Flowering Gene FT to from Cut Chrysanthemum‘Jinba’by Agrobacterium Mediate[J]. ACTA HORTICULTURAE SINICA, 2010, 37(3): 441-448.

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