油桃芽高温处理后酚和活性氧与休眠解除的关系

园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 963-968.

油桃芽高温处理后酚和活性氧与休眠解除的关系

王海波^{1, 2, 3};王孝娣2;高东升^1*;李疆³

(¹山东农业大学园艺科学与工程学院, 山东泰安271018; ² 中国农业科学院果树研究所, 辽宁兴城125100; ³新疆农业大学园艺学院, 新疆乌鲁木齐830052)

收稿日期:2006-01-18 修回日期:2006-09-19 出版日期:2006-10-25 发布日期:2006-10-25

Relationships of Total Phenolics, Reactive Oxygen Species and DormancyRelease in Nectarine Bud Treated by Heating

Wang Haibo^{1, 2, 3};Wang Xiaodi²; Gao Dongsheng^1*;Li Jiang³

(¹College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China;² Fruit Research Institute, Chinese Academy of Agricultural Science, Xingcheng, Liaoning 125100, China; ³College of Horticulture, Xinjiang Agricultural University, Urmuqi, Xinjiang 830052, China)

Received:2006-01-18 Revised:2006-09-19 Online:2006-10-25 Published:2006-10-25

摘要/Abstract

摘要： 以6年生曙光油桃/青州冬雪蜜桃为试材, 研究40℃、45℃、50℃高温短时间处理对油桃芽总酚含量、活性氧含量及相关酶活性和自然休眠解除的影响。结果表明: 40℃高温处理对油桃芽自然休眠的解除具有负调控效应(11月30日) 或效应不明显( 12月10日) , 其萌芽级数、·OH (羟基自由基)和O₂· (超氧阴离子自由基) 产生速率、H₂O₂ (过氧化氢) 含量及POD (过氧化物酶) 、CAT (过氧化氢酶) 、PPO (多酚氧化酶) 活性均低于对照(11月30日) 或与对照无显著差异( 12月10日) , 而总酚含量和PAL (苯丙氨酸解氨酶) 、SOD (超氧化物歧化酶) 活性高于对照(11月30日) 或与对照差异不明显(12月10日) ; 45℃和50℃高温处理对油桃芽自然休眠的解除呈正调控效应, 其萌芽级数、·OH和O₂· 产生速率、H₂O₂含量及POD、CAT、PPO活性与对照相比明显升高, 而总酚含量和PAL、SOD活性显著降低,虽然部分花芽和叶芽因高温处理而死亡, 但是在存活芽中短时间高温对油桃芽自然休眠解除的促进作用明显增强。总酚含量的降低和活性氧的迅速增加可能是高温解除自然休眠的原因。

关键词: 油桃, 芽, 高温, 自然休眠, 酚类物质, 活性氧代谢

Abstract: Effects of short-term heating at 40℃, 45℃ and 50℃ on bud livability, bud burst, the content of reactive oxygen and total phenolics, and the activity of related enzyme in‘Shuguang’nectarine bud were studied in order to investigate the mechanism of short-term heating releasing the endodormancy. The results indicated that effects of short2term heating on the endodormancy were differentwith date, temperature and continuous time of treatment. On November 30, compared with no2heating treatment ( control) , the date of
endodormancy release was postponed, the bud burst series and the rate of production in O₂· and ·OH, the content of H₂O₂ and the activity of CAT, POD, PPO enzyme were lower, and the content of total phenolics and the activity of PAL, SOD enzyme were higher on 40℃-heating treatment. Whereas, 45℃ and 50℃-heating treatment had the contrary effects. On December 10, 40℃-heating treatment nearly had the same results as control on the above factors, and the effects of 45℃ and 50℃-heating treatment on endodormancy release
were the same as the treatment on 30 November, and the effect of the former was superior to the latter. The decrease of the total phenolics content and the significant increase of reactive oxygen content and production rate were probably the cause of endodormancy release.

Key words: Nectarine, Bud, Heating, Endodormancy, Total phenolics, Reactive oxygen species

王海波;;;王孝娣;高东升;李疆. 油桃芽高温处理后酚和活性氧与休眠解除的关系[J]. 园艺学报, 2006, 33(5): 963-968.

Wang Haibo;;;Wang Xiaodi;Gao Dongsheng;Li Jiang. Relationships of Total Phenolics, Reactive Oxygen Species and DormancyRelease in Nectarine Bud Treated by Heating[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 963-968.

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