海南部分荔枝种质的RAPD分析

园艺学报 ›› 2004, Vol. 31 ›› Issue (2): 224-226.

海南部分荔枝种质的RAPD分析

陈业渊;邓穗生;张欣;魏守兴;高爱平;王家保;周斌

(中国热带农业科学院品种资源所，儋州 571737；中国热带农业科学院植保所，儋州571737；华南热带农业大学园艺系，儋州 571737；海南琼山水果所，海口 570000)

收稿日期:2003-06-10 修回日期:2003-10-08 出版日期:2004-04-25 发布日期:2004-04-25

RAPD Analysis of Genetic Relationship among Partial Litchi Germplasms inHainan Island

Chen Yeyuan;Deng Suisheng;zhang Xin;Wei Shouxing;Gao Aiping;Wang Jiabao;Zhou Bin

( Germplasm Research Institute of Tropical Crops， Plant Protection Research Institute of Tropical Crops，Chinese Academy of Tropical Agricultural Sciences，Danzhou 571737，China； Department ofHorticulture，South China University of Tropical Agriculture，Danzhou 571737，China ；Qiongshan Research Institute ofFruit，Haikou 570000，China )

Received:2003-06-10 Revised:2003-10-08 Online:2004-04-25 Published:2004-04-25

摘要/Abstract

摘要： 采用10个10碱基的随机引物，通过RAPD分析的方法，在分子水平上对海南30份荔枝种质
进行鉴定、分类和亲缘关系分析。结果表明，大多数种质相似系数在55% ～80%之间，说明他们之间的亲缘关系较近；在相似系数为0.61的水平上，30份海南荔枝种质被分为4组，与以龟裂片特征为主要分类依据的形态分类有一定的相关性，但是RAPD分类比形态分类的分类范围更细。因此，传统形态分类不能完全反映出品种间的亲缘关系，有待结合RAPD分析方法进一步完善。

关键词: 荔枝, RAPD标记, 聚类分析, 亲缘关系

Abstract: Identification．classification and genetic relationship of 30 litchi germplasms of Hainan island were measured at the molecular level by random amplified polymorphic DNA (RAPD)with ten arbitrary primers of 10 bases． The results revealed that similarity coefficients of most of litchi germplasms were among
55%—8O%．Which indicated that the genetic relationships between them were closer related． 30 litchi germplasms were divided into 4 groups at the level of the similarity coemcient of 61% ．Although a certain correlation with the morphological classification based mainly on the characteristic of tubercles on the ripe fruit skin，the range of classification by RAPD was more detailed than that of morphological classification．It is concluded that the traditional morphological classification system can not reflect fully the genetic relationships between litchi germplasms，and need further improvement according to the result by RAPD analysis．

Key words: Litchi, RAPD marker, Cluster analysis, Genetic relationship

中图分类号:

S667．1

陈业渊;邓穗生;张欣;魏守兴;高爱平;王家保;周斌. 海南部分荔枝种质的RAPD分析[J]. 园艺学报, 2004, 31(2): 224-226.

Chen Yeyuan;Deng Suisheng;zhang Xin;Wei Shouxing;Gao Aiping;Wang Jiabao;Zhou Bin. RAPD Analysis of Genetic Relationship among Partial Litchi Germplasms inHainan Island[J]. ACTA HORTICULTURAE SINICA, 2004, 31(2): 224-226.

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